AUTOMATED CHARACTERIZATION OF TINY VESICLES

Miltenyi Biotec has extended its portfolio with the MACSPlex Exosome Kit, human. The fast and efficient process enables the characterization of 37 exosome surface markers in a single experiment.

Studies indicate that the physiological significance of exosomes is not to be ignored. The molecular content that is passed from one cell to adjacent cells or the microenvironment via the tiny vesicles can transport important information and indeed play a role in the pathophysiology of the whole organism. This is why it is of great interest to develop methods that make it possible to experimentally characterize exosomes. Miltenyi Biotec’s new MACSPlex Exosome Kit, human, enables the qualitative as well as the semi-quantitative analysis of 37 exosome-specific surface markers.

Miltenyi Biotec has developed the MACSPlex Exosome Kit for the automated characterization of exosomes using flow cytometry. The screening approach is based on fluorescent immune conjugates, the MACSPlex Exosome Capture Beads, which are included in the kit and bind different exosome-specific epitopes. The MACSPlex Exosome Detection Reagent marks the bound exosomes with the pan-exosome markers CD9, CD63, and CD81. The result is a sandwich complex, consisting of the MACSPlex Exosome Capture Bead, the exosome and the detection reagent, which can subsequently be analyzed based on the respective fluorescent properties. Then, using flow cytometry, one can obtain semi-quantitative data on 37 exosomal surface epitopes.

The ideal counterpart to Miltenyi Biotec’s MACSPlex Exosome Kit, human would be a compatible flow cytometer such as the MACSQuant® Analyzer. Using this combination, flow cytometry can be carried out in a fully automated 96-well high-throughput format resulting in a fast and efficient analysis.