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avatar: tdmur
tdmur
3 posts
Replied to tdmur's post on February 26th, 2018

Hello,

Our lab recently acquired a 6500 and we're attempting to get it up and running. The lead shielding and PMT were removed by a technician prior to moving the instrument and we believe we've put everything back together correctly. Our issue is that when we attempt to count a sample (or standard) we receive the error message, "INVALID SAMPLE COUNT H# ABORT COUNT RATE TOO LOW" and we're not sure what else to try. I've included some pictures for reference.

Any ideas?

https://i.imgur.com/RSsDySw.jpg

https://i.imgur.com/3mGlLjz.jpg

 

avatar: dpkleessr
dpkleessr
1138 posts
Replied to tdmur's post on February 26th, 2018

Your issue could be a number of things.  Are you positive that you have reconnected everything?  Yeah, I know that you think it is a dumb question but it would not be the first time I encountered it during my career with Beckman Coulter.  You 137Cesium source may have come loose in its spring, you may also have a defective PMT, PMT preamp, or there may now be a problem with the front end board that the PMT's connect to.  There is a limited amount of troubleshooting that a user can do without a service diagnostic card which all BCI service engineers carry but I believe that a service visit is required.

Don

avatar: tdmur
tdmur
3 posts
Replied to dpkleessr's post on February 26th, 2018

Not a dumb question at all as we initially missed a few connections the first time through. I believe we have everything hooked up correctly now but we did notice that the diaphragm "shutter" appears to be in it's closed position around the rod that runs through the PMT. Is it possible to release the shutter so we can remove the rod/plunger? And could it be that the shutter is sticking closed and not allowing the sample to come up into the tube all they way?

Thanks for your help!

avatar: dpkleessr
dpkleessr
1138 posts
Replied to tdmur's post on February 26th, 2018

No way for you to do what you want without a diagnostics card.  If the diaphragm was part of your problem then you would have received a diagnostic message telling you it had a problem either opening or closing.  The "rod" is actually called the lead plug and it is what keeps your samples upright and centered within the detector housing.  You do want to be careful exposing the PMT's to sun or fluorescent lighting as it could take overnight until they quiet down enough to get even close to normal readings and if they have been light shocked then that could also cause your problem.

Don

avatar: tdmur
tdmur
3 posts
Replied to dpkleessr's post on February 27th, 2018

Thanks for the info, Don. Looks like we may just need to schedule a visit with our service rep. I'll update this thread once we get the issue resolved.

avatar: dpkleessr
dpkleessr
1138 posts
Replied to tdmur's post on February 27th, 2018
I hope that they can resolve it for you but be aware, just about all of the parts I listed carried a hefty price tag from Beckman. I have no idea what any 3rd party service group would charge for those same parts. Don
avatar: tfisc
tfisc
2 posts
Replied to dpkleessr's post on July 2nd, 2020

Hey!

We have the same problem with our Beckman counter now as well, so I wanted to ask, if you could resolve the problem?

Our counter worked the whole time but from one day to the other it started giving the error message described above.

Thank you for your help!

avatar: brenbobb01
brenbobb01
7 posts
avatar: bobbrencher01
bobbrencher01
7 posts
Replied to brenbobb01's post on July 14th, 2020

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avatar: colinh
colinh
3 posts
Replied to bobbrencher01's post on July 24th, 2020

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