Amaxa Biosystems Nucleofector® II


Nucleofector® II

Amaxa® Nucleofector® Technology is a highly efficient non-viral method for transfection. It is based on 2 components: the Nucleofector® Device that delivers unique electrical parameters, and Nucleofector® Kits. These contain Lonza certified cuvettes, pipettes, Nucleofector® Solution and Supplement. Each solution and supplement is individually developed for every primary cell type. For cell lines, five different Nucleofector® Solutions (C, L, R, T and V) are available.

The electrical parameters of the Nucleofector® Device are different from any other commercially available electroporation device. Each electrical setting is displayed as a distinct program which has been adapted to the requirements of a particular cell type. As the electrical settings are pre-programmed into the Nucleofector® Device, optimization of the electrical parameters by the user is not necessary.

The device weighs only 2.6 kg and is conveniently sized so that it easily fits under a sterile hood, which greatly facilitates sterile work with cells.


  • High efficiencies (up to 90% gene transefer efficiency with high cell viabilty and up to 99% transfection efficiency with siRNA duplexes even in suspension cells)
  • Fast experiments (expression within hours - from transfection to analysis in one day)
  • Easy handling (motor-driven carousel for automatic cuvette handling)
  • High convenience (large graphic display for easy day-to-day use)
  • Great flexibility (softwar enables the filing of individual programs)
  • Future-proof (extended program filing capacity)


Amaxa® Nucleofector® II

Amaxa® Nucleofector® II

Manuals and Resources

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Instrument Documents

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Amaxa Biosystems was acquired by Lonza Group    Amaxa Biosystems GmbH develops and commercializes gene transfer technologies and products....

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Questions and Answers

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Cells lines and specific kits

Hello, I would like to know that, for transfering the following cell lines, which kit do we need to order? (1) NK92 (2) Jurkat (3) RBL-2H3

Nucleofection of neonatal rat cardiomyocytes

Hi,   I tried to nucleofect neonatal rat cardiomyocytes with your kit (Amaxa-Rat Cardiomyocyte-Neonatal Nucleofector). I follow the protocol step by step but unfortunately, 12 hours after the nucleofection, almost all my cells died...

Can mouse fertilized eggs be electroporated by Amaxa?

I want to transfect genes into mouse fertilized eggs. Could you please to tell whether and how genes can be transfect into mouse fertilized eggs by Amaxa?

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Amaxa Nucleofector 96-Well Shuttle System w WARRANTY

Price: $2,750


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