Hitachi Medical Systems Fluorescence F-2500


Fluorescence F-2500

The F-2500 Fluorescence Spectrophotometer has been designed to include software and performance features that address the widest range of fluorescence applications with scan speeds of up to 3000 nm/min, resolution of 2.5 nm and comprehensive data processing functions for photometry, wavelength scanning and time scanning. Applications include life sciences, biotechnology, quality control, education, new materials and other basic scientific researches.     This model has been replaced with the F-2700

* This item has been discontinued by the manufacturer.

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Hitachi F-2700

Fluorescence F-2500
Additional Specifications
  • Minimum Sample Requirement: 0.6 mL (in use of standard 10mm cell),   
  • Photometric Principle: Monochromatic light monitoring with ratio calculation, Light Source: 150W Xenon lamp,   
  • Wavelength Range: 220 to 730 nm, and zero-order light,   
  • Slit Width: 2.5, 5, 10, 20 nm,   
  • Resolution: 2.5 nm,   
  • Wavelength Accuracy: +/- 3.0 nm,   
  • Wavelength Scan Speed: 15, 60, 300, 1500, 3000 nm/min,   
  • Wavelength Drive Speed: 12,000 nm/min,   
  • Photometric Value Display Range: 0.000 to 9999,   
  • Dimensions: 500W x 630D x 270H mm,   
  • Weight: 35 kg,   
  • Power Requirements: 100, 115, 220, 230, 240V AC, 50/60Hz, 400VA,   
  • Sensitivity: S/N-800 or better (RMS),S/N-450 or better (peak to peak), .  


  • Delivers excellent sensitivity two times higher than the conventional model. (S/N Ratio: 450 or better)  
  • Incorporates a multi-step slit of 2.5 to 20 nm, making it applicable to a variety of samples  
  • PC control using FL Solutions® software featuring versatile quantitative analysis functions, data export function into Excel, and print preview function  
  • Sophisticated electronics make it easy to find out the quantitative determination of a sample (of unknown concentration) by preparing a linear calibration curve in a concentration range on the order of approx. six digits  
  • True spectra measurable without an instrumental response (Spectral correction) OPTIONAL  
  • Easy to control with a mouse in an icon-based operating environment.  
  • Horizontal beams permit measuring a trace amount of sample  


  • Model F2500

Manuals and Resources

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What is the Calibration procedure for the f-2500?

Wavelength calibration of an F-2500

Does anyone know how to calibrate the wavelength on an F-2500?  From spectra of quinine sufate in H2SO4(aq) my emission peak is shifted about 4nm off from the literature values.  I assume I need to change the grating angle on the emission...


Slit Width: 2.5, 5, 10, 20 nm. Probrably these values are wrong and should be  given in micrometers, not in nanometers for the possible slits in the F-2500 fluoremeter. Am I correct!?  

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