Varian Cary 50

Cary 50

The award-winning, budget priced Cary 50 makes it possible for all users to experience the power of a Cary.

The innovative design of the Cary 50, which incorporates a Xenon flash lamp, enables it to offer many advantages over traditional UV-Vis spectrophotometers.

The Cary 50 is controlled by the new Cary WinUV software. This Windows based software features a modular design which makes it easy to use. And of course, the Cary 50 can be fitted with the full range of accessories to provide extra capabilities.

Instrument Attributes
Height	205 mm
Depth	590 mm
Width	500 mm
Power Supply	Instrument draws maximum of 26 W of power from the host PC power supply. The power rating is +5 V DC <1 A, +12 V DC <1.5 A, -12 V DC <0.25 A. The Cary 50 interface card fits into a standard ISA slot in the host PC and requires a standard PC internal hard disk power supply connector. Operation of motor driven accessories may increase the +12 V requirement by a further 2 A (24 W maximum). The host PC must be certified to standard IEC 60950 or equivalent.
Weight	21 kg
Stray Light	At 198 nm (12 g/L KCl, TGA & BP/EP method):
Wavelength Range	190–1100 nm
Operating Conditions	Below 853 m altitude: 10–35 °C, 8–80% relative humidity, non-condensing. Between 853 and 2133 m altitude: 10–25 °C, 8–80% relative humidity, non-condensing
Bandwidth	Fixed at 1.5 nm
Scanning Speed	Maximum 24000 nm/min
Detector	2 silicon diode detectors
Optical System	Czerny-Turner monochromator
Photometric Range	± 3.3 Abs
Sample Compartment	(WxDxH): 130 mm x 523 mm x 123 mm Note that sample compartment can be left open during measurement due to room light immunity of Cary 50.
Additional Specifications	Grating: Holographic, 27.5 x 35 mm, 1200 lines/mm, blaze angle 8.6° at 240 nm Beam splitting system; Beam splitter UV-Vis limiting resolution: = 1.5 nm Wavelength accuracy: ± 0.5 nm at 541.94 nm Wavelength reproducibility: ± 0.1 nm Photometric accuracy (Abs): Using NIST 930D filters at 1 Abs ± 0.005 At 0.2, 0.5 & 0.75 Abs (14.2% w/v KNO3, TGA method) ± 0.01 0.292 to 0.865 Abs (60.06 mg/L K2Cr2O7, BP method) ± 0.01 Photometric display: ± 9.9999 Abs, ± 200.00 %T Photometric reproducibility (Abs): Using NIST 930D filters, at 465 nm, 2 s SAT Maximum deviation at 1 Abs < 0.004 Standard deviation for 10 measurements < 0.00050 Using NIST 930D filters, at 546.1 nm, 2 s SAT Maximum deviation at 0.5 Abs < 0.003 Standard deviation for 10 measurements < 0.0030 Photometric stability (Abs/hour): After 30 minute warm up, 500 nm, 10 s SAT < 0.0004 Photometric noise (Abs, RMS): 500 nm, 1 s SAT At 0 Abs < 0.0001 At 1 Abs < 0.0005 At 2 Abs < 0.005 260 nm, 1 s SAT At 0 Abs < 0.00015 Baseline flatness (Abs): 200 to 850 nm, smooth 21 filter applied, baseline corrected ± 0.001 Access: Top and front Signal averaging: 0.0125 to 999 seconds Slew rate: 24000 nm/min Data interval: 0.15–5.0 nm Repetitive scanning: 4800, Maximum number of cycles: 999, Maximum cycle time (min): 9999 Data collection rate: (kinetic studies) points per min per cell 1 cell = 4800, 6 cell = 6, 12 cell = 3, 18 cell = 2 6 cells, 0.0375 SAT 0.38 s dwell time = 40 to 50 12 cells, 0.0375 SAT 0.38 s dwell time = 20 to 30 18 cells, 0.0375 SAT 0.38 s dwell time = 10 to 20 Temperature monitors: Temperature probe inside cuvette (using the Temperature Probe Accessory) Minimum sample volume: Approximately 5.0 µL Warranty: Twelve (12) months, though this may vary according to locations.

Features

The maximum scan rate is 24 000 nm per minute. That means you can scan the whole wavelength range of 190-1100 nm in less than 3 seconds.
With a data collection rate of an impressive 80 points per second you'll have all the information you need about your kinetics assay.
The Cary 50 can measure samples up to 3 Abs so you won't have to dilute as often.
The Xenon lamp flashes only when acquiring a data point, unlike a diode array which exposes the sample to the whole wavelength range with each reading, causing degradation of photosensitive samples.
As the Xenon lamp is very intense, the Cary 50 can use a beam splitter without the loss in energy causing excessive photometric noise. The beam splitter allows simultaneous reference beam correction, so peaks will not shift as the scan speed changes.
The Cary 50's super-concentrated beam makes it ideal for fiber optic work.
The light beam is narrow and very intense, so even if you are using microcells you will still get excellent noise performance.
The Cary 50 is unaffected by room light. You can operate with the sample compartment open or closed, you won't notice the difference.