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Thermo Scientific Pierce Binding and Elution Buffers for IgG Purification

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Pierce Binding and Elution Buffers for IgG Purification

These products include IgG Binding Buffers specific for each immunoglobulin-binding protein (Protein A, Protein G, Protein A/G). The Pierce IgG Elution Buffer provides efficient dissociation and elution of antibodies from all three IgG-binding proteins, including Protein L. This low-pH elution buffer is also effective for most antibody-antigen or protein-protein affinity purification systems.     
     
The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.     
     
Finally, the Mouse IgG1 Mild Elution Buffer, when used in combination with Protein A resin, enables selective elution and separation of the IgG1 subclass from total bound mouse IgG.

Models

Protein A IgG Binding Buffer
Attributes

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Additional Specifications Quantity: 1 L OR 3.75 L
Sufficient For: Maximum binding of IgG to Protein A resin
Formulation: Proprietary buffer (pH 8) with EDTA and glycerol
Protein G IgG Binding Buffer
Attributes

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Additional Specifications Quantity: 1 L OR 3.75 L 
Sufficient For: Maximum binding of IgG to Protein G resin 
Formulation: Proprietary buffer (pH 5) with sodium azide
Protein A/G IgG Binding Buffer
Attributes

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Additional Specifications Quantity: 240 mL
Sufficient For: Maximum binding of IgG to Protein A/G resin
Formulation: Proprietary buffer (pH 8) with EDTA
IgG Elution Buffer
Attributes

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Additional Specifications Quantity: 1 L OR 3.75 L
Sufficient For: High-yield dissociation of IgG from affinity resins
Formulation: Proprietary amine-buffer (pH 2.8)
Gentle Ag/Ab Binding Buffers
Attributes

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Additional Specifications Gentle Ag/Ab Binding Buffer, pH 8.0:
Quantity: 1 L OR 3.75 L
Sufficient For: Phosphate-free binding of IgG for direct compatibility with Gentle Ag/Ab Elution Buffer
Formulation: Sodium borate buffer (pH 8)

Gentle Ag/Ab Elution Buffer, pH 6.6:
Quantity: 500 mL OR 3.75 L
Sufficient For: Near-neutral elution of IgG
Formulation: Proprietary, high-molarity buffer (pH 6.55)
Mouse IgG1 Mild Elution Buffer
Attributes

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Additional Specifications Quantity: 500 mL
Gentle Ag/Ab Buffer Kit
Attributes

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Additional Specifications Quantity: 200 mL kit
Sufficient For: Binding and near-neutral elution of antibodies with Protein A or G
Kit Contents: Pierce Gentle Ag/Ab Binding Buffer(100mL) & Pierce Gentle Ag/Ab Elution Buffer (100mL)

Features

         
  • Three optimized binding buffers – for Protein A, Protein G or Protein A/G     
  • Two elution buffer options – acidic (pH 2.8) or high salt (near-neutral)     
  • Special system for mouse IgG1 – selective, two-stage elution from Protein A agarose     
  • Kits contain both binding and elution buffers     
         
    Item-Specific Features:     
  • Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA     
  • Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA     
  • Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide     
  • IgG Elution Buffer: pH 2.8; amine-based     
  • Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide     
  • Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)     
  • Mouse IgG1 Mild Elution Buffer: pH 6.0; contains 0.02% sodium azide     

Photos

Pierce Antibody Binding and Elution Buffers

Pierce Antibody Binding and Elution Buffers

Manuals and Resources

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