STEMCELL Technologies Inc. SepMate



SepMate™ is a tube that facilitates the isolation of PBMCs or specific cell types by density gradient centrifugation. SepMate™ tubes contain an insert that provides a barrier between the density gradient medium and blood. SepMate™ eliminates the need for careful layering of blood onto the density gradient medium, and allows for fast and easy harvesting of the isolated mononuclear cells with a simple pour. SepMate™ is also compatible with RosetteSep™ enrichment cocktails, allowing isolation of specific cell types in less than 30 minutes.
Part Number 15450 (20 tubes), 15460 (100 tubes)
Construction Polypropylene
Additional Specifications Size: 50 mL 
Recommended for: The isolation of PBMC from whole blood using density gradient medium centrifugation & the isolation of specific cell types when used with RosetteSep™ cell enrichment cocktails   
Cell Type: B cells, CD4+ T cells, CD8+ T cells, Dendritic cells, Granulocytes & subsets, Lymphocytes, Monocytes, Myeloid cells, NK cells, Other T cell subsets, Regulatory T cells, T cells   
Selection Method: Negative   
Species: Human   
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.


  • Eliminates the need for carefully layering blood over the density gradient medium (e.g. Ficoll-Paque™, Lymphoprep™)   
  • Reduces total centrifuge time to 10 minutes with the brake on   
  • Allows fast and easy harvesting of the isolated mononuclear cells by simply pouring off the supernatant   
  • Can be combined with RosettSep™ enrichment cocktails to isolate specific cell types in just 25 minutes   


SepMate™-50 Tubes

SepMate™-50 Tubes

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Using SepMate on Cord Blood

When using a standard 50 ml conical tube with ficoll as the density medium, there are always nucleated RBCs that colocalize with MNCs at the ficoll/plasma interface. Will SepMate prevent this contamination of these nucleated RBCs in the buffy layer?

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