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BioTek ELx50

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ELx50

The ELx50 Washer is now a truly flexible platform that provides washing capabilities unsurpassed in its class. Newly available models with biomagnetic separation and vacuum filtration capabilities make the ELx50 an excellent choice for automating the wash steps of 96-well magnetic or polystyrene bead assays, such as those developed on the Luminex xMAP® technology platform. Users not requiring an integrated stacker can expect the same great bead washing performance as BioTek’s industry leading full plate washers but here in a more compact footprint. Comprehensive onboard software makes programming quick and easy. Custom magnets, in either flat or ring designs, incorporate high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer magnetic beads with superior retention. A fast and efficient vacuum filtration module allows vacuum to be adjusted with a range of settings for flexibility with polystyrene beads when using various filter pore sizes and sample viscosities. The vacuum filtration module is also well suited for filtration-to-waste processes such as PCR cleanup after DNA amplification to remove unwanted residues or reaction by-products with filtrate. The washer’s modular platform allows for processing standard solid bottom plates used in a laboratory’s many traditional ELISAs and cell based assays.

The ELx50 can also be equipped with our patented Dual-Action™ 16-channel manifold. This breakthrough design allows for independent control of the dispense and aspiration manifolds for overfill washing and overflow protection in both 96- and 384-well formats. The problem of fitting dispense and aspirate tubes into a much smaller 384-well is solved with the ELx50. Additionally, in a 96-well format, the Dual-Action manifold provides incomparable wash performance with two sets of dispense and aspiration tubes per well. This exclusive method of two-fold aspiration guarantees low residuals.

As a welcome upgrade from manual processing, the new ELx50 Microplate Strip Washer will bring to your lab an all inclusive wash solution offering consistent performance and unattended operation.
ELx50
Attributes
Shaking Pattern Programmable in minutes and seconds up to 60 minutes Intensities - slow, medium, fast or variable
Depth 16 in
Height 6.5 in
Width 14 in
Power Requirements Compatible with 100 to 240 V~ ± 10% @ 50-60 Hz
Weight 22 lbs
Additional Specifications Assays Magnetic bead, polystyrene bead (model dependent)
  • Multiplex assays
  • Bead-based ELISA

ELISA
Cell-based assays
Protein arrays
Filtration-to-waste processes (model dependent)
Separation: Biomagnetic separation, vacuum filtration (model dependent)
Microplate Types: 96- and 384-well (model dependent)
Standard height and low profile
Solid and filter bottom (model dependent)
  • Filter pore sizes 0.45 µM to 1.2 µM


Fluid Delivery: Internal positive displacement syringe drive
Soak Time: Programmable in minutes and seconds up to 60 minutes
Magnet: High strength 96-well format
Choice of two designs, custom for BioTek microplate washers:
  • Flat
  • Flat-bottom well - beads pulled to band across well bottom
  • Round-bottom well - beads pulled to button at well bottom
  • Ring - beads pulled to 4-zone ring at well bottom

Incorporated into carrier, removable for non-magnetic washing
Safety/Convenience/Maintenace:
Waste sensing, fluid detection (optional)
Aerosol cover
Overflow protection
Pre-programmed maintenance routines
Onboard Software:
2 x 24 character LCD display
25 alphanumeric soft keys
Create, edit or run multiple protocols
Operating Temperature: 15°C to 40°C (59° to 104° F)
Humidity: 10% to 85%, non-condensing

Washing
Manifold Type:
96-/384-well washing:
Dual-Action™ 16-tube (1x16) manifold

96-well washing:
8-tube (1x8) manifold
12-tube (1x12) manifold
Washing Speed:
Solid bottom plates
3 asp. / disp. cycles: 300 µL/well, 96 wells, 8-tube manifold, final aspirate: < 130 seconds
Filter bottom plates:
Variable, based on wash parameters
Vacuum Filtration: Selectable vacuum levels (final at 30 seconds):

0.45 µM:
Low: -91mmHg
Medium: -150 mmHg
High: -313 mmHg

1.2 µM:
Low: -95 mmHg
Medium: -155 mmHg
High: -299 mmHg
Vacuum filtration time range: 5 to 999 seconds
Volume Range: 25 - 3,000 µL/well (model dependent)
Selectable in 1 µL increments
Buffer Selection: Automatic switching for up to 3 wash buffers (model dependent)
Flow Rates: 1 - 9
Wash Cycles: 1 - 10
Dispense Precision: = 3% CV
Residual Volume:
Solid Bottom Plates
= 2 µL/well
Filter Bottom Plates
Average increase weight of plate = 1.2 g after dispensing 300 µL to 0.45 µm plate, filtration 30 seconds, low vacuum, blotted
Sterilization:
Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Supply Bottle Volume: 2 L
Physical Characteristics

Regulatory: All BioTek microplate instrumentation is CE and ETL marked.

Features

General:
  • Syringe drive fluid delivery system for precise control over all fluid flow rates
  • Wash single strips or full plates
  • 96- and 384-well microplates
  • Unique priming trough built into removable microplate carrier for effortless priming and easy maintenance
  • Automatic buffer switching
  • Create, edit, delete and copy up to 75 programs from the keypad
  • Link up to 10 programs to run the most complex routines
  • Multi-user friendly; total programming control for complex research applications, yet easy to use for routine clinical work
  • Programmable shaking duration and intensity
  • Built-in maintenance programs
  • Bottom washing and crosswise aspiration protocols
  • Small footprint with integrated dispense and waste pumps
  • Aerosol cover for safety

Biomagnetic Separation
  • High strength biomagnetic separation for super paramagnetic bead based assays, such as Luminex xMAP®
  • High energy neodymium iron boron magnets
  • Optimized field gradients within wells
  • Choice of flat or ring magnets - two 96-well high strength designs custom for BioTek microplate washers
  • Rapid separation of micrometer and nanometer beads
  • Superior microsphere retention

Vacuum Filtration
  • Fast and efficient vacuum filtration for polystyrene bead assays or filtration-to-waste processes
  • Range in accommodated filter pore sizes from 0.45 mm to 1.2 µmm
  • Selectable vacuum levels from –91 mmHg to –313 mmHg for varying sample viscosities
    Carrier vacuum grate directs liquid droplets away from plate for minimized after-wash residual on plate’s underside and no cross contamination
    Software programmable dispense volume, soak and aspiration time for completely unattended operation and recall

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Manuals and Resources

Note: We appreciate the support of individuals and companies making their documents and resources available to our community. If you find a document or resource that does not belong please let us know by emailing info@labwrench.com

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contamination trouble

hi > the problom I have contamination effacting my result , in BIOTEK ELx 50 microwell wosher. how do I solve this problom?

ELx50 and EIA

Currently I am trying to use the ELx50 to take the place of hand washing wells from an EIA kit. The plate is read using the Bio Tek ELx800. The kit calls for interpretation of results to be read spectrophotometricly with a positive reading being >=0...

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