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BERTHOLD TECHNOLOGIES bScreen LB 991 Label-free Microarray Reader

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bScreen LB 991 Label-free Microarray Reader

The bScreen is a unique instrument for biomolecular interaction studies. The bScreen LB 991 combines the performance of µArray formats with the information available from label-free technologies. With a footprint of only 66 x 61 cm2 it substitutes a fluorescence µArray reader and a conventional label-free system (e.g. SPR) in a single instrument. The exclusive Biametrics 1Λ-Reflectometric Interference Detection (1Λ-RIDe) facilitates the analysis of the complete binding kinetics of up to 10,000 individual interactions in one single measurement for real high-throughput screening (rHTS).
bScreen LB 991 Label-free Microarray Reader
Attributes
Additional Specifications Sample Types:  
LOW MW drug candidates (MW < 150 Da) up to high MW proteins, DNA, RNA, polysaccharides, lipids, cells, viruses, nanoparticels in various matrices (DMSO-containing buffers, urin 
Sample Format: 
Array chips in standard microscopy slide format with specialized biopolymer coating 
Compatible to most µArray spotters 
Typical Throughput:  
Up to 240,000 interactions/24 h (Direct binding assay up to 10,000 targets per assay, assay time 1 h), 
Antibody screening: 240,000 samples / 24 h 
Typical Evaluation Time:  
Screening: < 1 h; Kinetics: 1 to 2 h 
Measurement Parameters/Information:  
Kinetic and affinity data (KD, ka, kd), specifity, concentration, thermodynamic parameters (ΔH, ΔS) 
Detection Limits:  
< 1pg/mm2 
Rate Constants: 
ka: 103 - 107 M-1s-1 (higher for macromolacular analytes) 
kd: 10-6- 0.5 s-1

Features

1Λ-RIDe Technology       
The patented 1Λ-RIDe technology by our partner Biametrics analyses changes in the optical thickness (n x d) due to specific analyte binding to a bio-functionalized array chip. The measurement is accomplished by detecting the light intensity resulting from the interference of light beams reflected at various surface layers of the sample. This way, no additional staining is required and the binding of the analyte to the surface is monitored in a direct way. All measurements are carried out in a continuous sample flow, facilitating the complete characterization of the kinetics of the binding processes.       
       
The 1Λ-RIDe technology is largely temperature-independent, making expensive and time-consuming thermostatization of the instrument or the solutions obsolete.       
       
As a label-free method, the 1Λ-RIDe technology features a direct assay-format without the need of additional components (e.g. fluorescence-labelled antibodies). Thus material- and time intense washing steps inherent in established µArray protocols are not required. As a consequence, the overall quality of the analysis is increased, as the risk of dissociation of weak binders during the washing steps is prevented (false-negatives). Moreover, the 1Λ-RIDe technology does not suffer from the detection of false-positives due to unspecific binding of the fluorescence-labels to the surface       
       
Measurement Parameters       
  • Concentration (< 1 pg/mm2)       
  • Association rate constants ka (103 – 107 M-1s-1, higher for macromolecular analytes)       
  • Dissociation rate constants kd (10-6 – 0.5 s-1)       
  • Affinity constant KD       
  • Enthalpy (ΔH) and entropy (ΔS)       
           
    Sample Format       
    bScreen accepts conventional microscopy slides of 75 x 25 mm2 in size as array chips to guarantee full compatibility to almost all µArray spotters. This way, sample coverage or spotting patterns is not restricted to any pre-defined matrices and the user has complete freedom in the measurement layout. Do to the comprehensive compatibility to established µArray environments, also high-density arrays can be measured, opening the field of real high throughput screening (rHTS) for label-free applications.       
           
    Operating Software       
    The bScreen comes with an intuitive software package, which controls the setup-up of measurement protocols, the sample handling as well as the measurement procedure. Integrated interfaces enable the import of *.gal-files containing µArray-definitions for a rapid set-up of the sample area. The performant evaluation-kit enables comprehensive kinetic analyses of the measured data. Versatile export functions allow for the transfer of raw and measurement data to third party software for easy integration of the bScreen into LIMS-environments.
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