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Promega NanoBRET™ PPI Assays

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NanoBRET™ PPI Assays

The new BRET-based protein:protein interaction (PPI) assay uses NanoLuc® Luciferase as the BRET energy donor and HaloTag® protein, labeled with the NanoBRET™ 618 fluorophore, as the energy acceptor to measure the interaction of specific protein pairs. NanoBRET has been optimized for improved spectral overlap, increased signal, and lower background compared to conventional BRET assays providing a reproducible method for monitoring and screening protein interactions in live cells.   
   
Study both induction and inhibition of protein interactions in real time using full length proteins expressed at physiologically relevant levels creating PPI assays that allow you to assess true cellular protein targets.

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