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New England Biolabs NEBNext® Library Quant Kit for Illumina®

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NEBNext® Library Quant Kit for Illumina®

Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run. For Illumina sequencing specifically, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence performance. qPCR is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility than electrophoresis or spectrophotometry, which measure total nucleic acid concentration. Amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced.     
     
The NEBNext Library Quant Kit delivers significant improvements to qPCR-based library quantitation for next gen sequencing. The NEBNext Library Quant Kit for Illumina contains components that are optimized for qPCR-based quantitation of libraries prepared for Illumina next-generation sequencing platforms. The NEBNext Library Quant Kit contains primers which target the P5 and P7 Illumina adaptor sequences and a set of high-quality, pre-diluted DNA standards to enable reliable quantitation of diluted DNA libraries between 150–1000 bp.

Features

  • Provides more accurate and reproducible quant values than alternative methods and kits     
  • Compatible with libraries with a broad range of insert sizes and GC content, made by a variety of methods     
  • Requires only 4 standards, allowing more libraries to be quantitated per kit      
  • Supplied with a convenient, Library Dilution Buffer     
  • The NEBNext Library Quant Master Mix requires only the addition of primers     
  • Utilizes a single extension time for all libraries, regardless of insert size     
  • Library quant values can be easily calculated using NEB’s online tool, at NEBioCalculator.neb.com     
  • ROX is included in the kit, for use with qPCR instruments that require a reference dye for normalization
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    NE Quant Kit

    NE Quant Kit

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