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New England Biolabs WarmStart RTx Reverse Transcriptase

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WarmStart RTx Reverse Transcriptase

WarmStart RTx Reverse Transcriptase is a unique in silico designed RNA-directed DNA polymerase coupled with a reversibly-bound aptamer that inhibits RTx activity below 40°C. This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. RTx is a robust enzyme for RNA detection in amplification reactions and is particularly well suited for use in LAMP (loop-mediated isothermal amplification). The WarmStart property enables high throughput applications, room temperature setup, and increases the consistency and specificity of amplification reactions. RTx contains intact RNase H activity.
WarmStart RTx Reverse Transcriptase
Attributes
Additional Specifications Storage Temperature:     
-20°C     
Reaction Conditions:     
1X Isothermal Amplification Buffer     
Unit Definition:     
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 Μl in 20 minutes at 50°C using poly(rA)•oligo(dT)18 as template.

Features

  • RT-LAMP     
  • cDNA synthesis     
  • RT reactions requiring room temperature setup   
  • Inhibited RTx activity below 40°C   
  • Maximum activity at 55°C   
  • Sensitive detection with reduced false negatives from 100 ng all the way down to 1 pg of total input RNA   
  • Validated for RT-LAMP, RT-PCR and other RT reactions requiring room temperature setup.
  • Photos

    WarmStart RTx Reverse Transcriptase

    WarmStart RTx Reverse Transcriptase

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