Olympus FV1000



The Olympus FluoViewTM FV1000 is a next-generation imaging system designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging with minimal risk of damage to living specimens. In addition, the FV1000 offers a revolutionary synchronized laser scanning system called the SIM Scanner. While one laser stimulates, the second laser simultaneously provides high-resolution imaging. This coordination of laser stimulation and imaging makes the FV1000 an ideal choice for FRAP, FLIP and photoactivation.
Microscope Type Confocal
Additional Specifications Laser Light  
Ultraviolet/Visible light laser:  
LD laser: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635mW, 20mW  
Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe(G) laser (543nm, 1mW)  
AOTF laser combiner: Visible light laser platform with implemented AOTF system, Ultra-fast intensity modulation with individual laser lines, additional shutter control  
Continuously variable (0.1% - 100%, 0.1% increment), REX: Capable of laser intensity adjustment and laser wavelength selection for each region  
Fiber: Broadband type (400nm-650nm)  
Scanning and Detection  
Scanner module: Standard 3 laser ports, VIS - UV - IR  
Excitation dichromatic mirror turret, 6 position (High performance DMs and 20/80 half mirror), Dual galvanometer mirror scanner (X, Y)   
Motorized optical port for fluorescence illumination and optional module adaptation, Adaptation to microscope fluorescence condenser  
Detector module:  
Spectral Type Fluorescence Detection:  
Standard 3 confocal Channels (3 photomultiplier detectors)  
Additional optional output port light path available for optional units  
6 position beamsplitter turrets with CH1 and CH2  
CH1 and CH2 equipped with independent grating and slit for fast and  
flexible spectral detection  
Selectable wavelength bandwidth: 1-100nm  
Wavelength resolution: 2nm.  
Wavelength switching speed: 100nm/msec  
CH3 with 6 position barrier filter turret  
Filter Type Fluorescence Detector:  
Standard 3 confocal Channels (3 photomultiplier detectors)  
Additional optional output port light path available for optional units  
6 position beamsplitter turrets with CH1 and CH2  
CH1 to CH3 each with 6 position barrier filter turret  
(High performance filters.)  
Filters: High performance sputtered filters, dichromatic mirrors and barrier filters  
Scanning Method: 2 galvanometer scanning mirrors  
Scanning Modes:  
Pixel size: 64 x 64 — 4096 x 4096  
Scanning speed: 512 x 512 (1.1 sec., 1.6 sec., 2.7 sec., 3.3 sec., 3.9 sec., 5.9 sec., 11.3 sec., 27.4 sec., 54.0 sec.)  
256 x 256 bidirectional scanning (0.064 sec., 0.129 sec.)  
0.5 or 1 microsec dwell time per point for fast bidrectional scanning  
Spectral Type Fluorescence Detector: X,Y,T,Z,?  
Line scanning: Straight line with free orientation, free line, Point scanning  
Filter Type Fluorescence Detector: X,Y,T,Z  
Line scanning: Straight line with free orientation, free line, Point scanning  
Photo Detection Method: 2 detection modes: Analog integration and hybrid photon counting  
Spectral Type Fluorescence Detector: Single motorized pinhole  
pinhole diameter ø50 - 300µm (0.5µm step)  
Filter Type Fluorescence Detector: Single motorized pinhole  
pinhole diameter ø50 - 800µm (0.5µm step)  
Field Number (N.A.): 18  
Optical Zoom: 1x - 50x in 0.1x increment  
Z-drive: Integrated motorized focus module of the microscope, minimum increment 0.01µm or 10 nm  
Transmitted Light Detector Unit: Module with integrated external transmitted light photomultiplier detector and 100W Halogen lamp, motorized switching, fiber adaptation to microscope frame  
Motorized microscope: Inverted IX81, Upright BX61, Upright focusing nosepiece & fixed stage BX61WI  
Fluorescence illumination unit: External fluorescence light source with motorized shutter, fiber adaptation to optical port of scan unit  
Motorized switching between LSM light path and fluorescence illumination  
System Control  
PC: PC-AT compatible, OS: Windows XP Professional (English version), Memory: 2GB or larger, CPU:Pentium 3.2GHz or higher, Hard disk: 120GB or larger,  
Media: DVD Multi Drive, FV1000 Special I/F board (built-in PC), Graphic board: ATI RADEON X700 PRO  
Power Supply Unit:  
Spectral Type Fluorescence Detector: Galvo control boards, scanning mirrors and gratings, Real time controller  
Filter Type Fluorescence Detector: Galvo control boards, scanning mirrors  
Monitor: SXGA 1280X1024, dual 19 inch or larger monitors  
Optional Unit  
SIM Scanner: 2 galvanometer scanning mirrors, pupil projection lens, built-in laser shutter, 1 laser port  
Fiber introduction of near UV diode laser or visible light laser, Optional: 2nd AOTF laser combiner  
TIRFM unit: Available laser: 405~633nm. Motorized penetration ratio adjustment. Automatic optical setting for TIRFM objectives.  
4th CH detector: Module with photomultiplier detector, barrier filter turret, beamsplitter turret mounted with 3rd CH light path  
Fiber port for fluorescence: Output port equipped with FC fiber connector (compatible fiber core 100 -125µm)


Thanks to the variable VBF with 2 nm resolution, acquisition wavelength can be set freely to suit various fluorochromes. Simple separation of fluorescent cross-talk using the lambda scanning and unmixing functions.   
Laser power monitoring for stable stimulation. Stable excitation light via advanced laser intensity feedback system for greater stability. Measurement of fluorescent intensity during observation using live-plot.   
High Sensitivity   
Newly designed Analog Accumulation Circuit (AAC). Uniquely coated filters and dichromatic mirrors enhance sensitivity. Highly sensitive photomultiplier selected specifically for the FV1000. Superior optics with minimum optical return loss.




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FRET protocol

Hi - I am looking for a manual to measure acceptor photobleaching FRET by FV1000. Can anyone refer me to such document? Thanks

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