BioTek Lionheart™ FX Automated Live Cell Imager


Lionheart™ FX Automated Live Cell Imager

Lionheart™ FX Automated Live Cell Imager with Augmented Microscopy™ is an all inclusive microscopy system, optimized for live cell imaging with up to 100x air and oil immersion magnification. Brightfield, color brightfield, phase contrast and fluorescence channels offer maximum support for a wide range of imaging applications. A unique environmental control cover provides incubation to 40 °C and effective containment for CO2/O2 control. The available humidity chamber and reagent injector add a greater level of environment optimization for live cell imaging workflows. Gen5™ 3.0 software provides automated image capture and analysis, plus annotation and movie maker functions. Gen5 3.0 offers ease and simplicity across a broad range of live and fixed cell applications. Augmented Microscopy is the combination of all of these features in one compact system. With Lionheart FX, you can capture, analyze, annotate and produce videos with ease.
Lionheart™ FX Automated Live Cell Imager
Additional Specifications General     
Microplate types      
6- to 1536-well plates     
Other labware supported      
Microscope slides, Petri and cell culture dishes, cell culture flasks (T25, T75), counting chambers (hemocytometers), chamber slides     
Support for labware up to 1.5" tall     
Temperature control      
Incubation to 40 °C with optional environmental control cover     
Gen5 3.0 Data Analysis Software included     
Gen5 Image and Image+ Software available for advanced image analysis (option)     
CO2 and O2 control (option)      
0 - 20% CO2 control and 1 - 19% O2 control, with optional Gas Controller     
Environment control cover      
Top cover for light tight imaging and incubation control (option)     
Stage control      
High resolution lead screw driven stage with open access     
Humidity control      
Humidity chamber with rapid gas recharge (option)     
Imaging System     
Imaging mode      
Fluorescence, brightfield, color brightfield, and phase contrast     
Imaging method      
Single color, multi-color, montage, time lapse, Z-stacking, burst mode     
Light source      
High power LEDs (available wavelengths: 365 nm, 390 nm, 465 nm, 505 nm, 523 nm, 590 nm, 623 nm, 655 nm, 740 nm)     
16-bit gray scale, Sony CCD, 1.25 megapixel     
Camera binning      
Optional 2x2 binning for focus and/or image capture     
Camera exposure range      
5 milliseconds to 4 seconds     
Image outputs available      
Raw Images: 16-bit TIFF     
Saved Images: TIF, JPG, BMP, PNG, EMF, GIF     
Movies: MP4, WMV     
Objective capacity      
6 onboard, user-replaceable objectives     
Objectives available      
Air: 1.25x, NA .04; 2.5x (2.25x eff), NA: .07; 2.5x (2.75x eff), NA: .12; 4x, NA: .13; 10x, NA: .30;     
20x, NA: .45; 40x, NA.60; 60x, NA .70     
Oil: 60x, NA:1.42; 100x, NA: 1.40     
Phase objectives available: 4x, NA: .13; 10x, NA: .30; 20x, NA: .45; 40x, NA .60     
Image filter cube capacity      
Up to 4 onboard, user-replaceable cubes     
Imaging filter cubes available      
DAPI, CFP, GFP, YFP, RFP, Texas Red, CY5, CY7, Acridine Orange, CFP-YFP FRET, Chlorophyll, Phycoerythrin (PE), Propidium Iodide, CY5.5, TagBFP, GFP (Ex)-CY6 (Em), RFP (Ex)-CY5 (Em)     
Automated functions      
Autofocus, user-trained autofocus, auto exposure, auto-LED intensity     
Autofocus method      
Image-based autofocus     
Laser autofocus option     
Image collection rate      
Single Well Fastest Frame Rate Capture:     
*Full Resolution: up to 10 frames per second for single color images     
*2x2 Binning: up to 20 frames per second for single color images     
Microscope stage control      
Gen5 Software Control     
Optional joystick controller     
Reagent Injectors (Module Option)     
2 syringe pumps     
Supported labware      
6- to 384-well microplates, Petri and cell culture dishes, chamber slides     
Dead volume      
<1.65 mL with back flush     
Dispense tip options      
Aligned tip - aligned with optical path for dispensing for fast kinetic assays     
Offset tip - dispensing is offset from the optical path     
Dispense volume      
5 - 1000 µL in 1 µL increment     
Dispense accuracy      
± 1 µL or 2%     
Dispense precision      
≤ 2% at 50 - 200 µL     
Physical Characteristics     
250 Watts max.     
With cover closed or without cover: 18.3" D, 17.9" W, 14.1" H (46.5 cm x 45.5 cm x 35.8 cm)     
With cover fully open: 18.3" D, 17.9" W, 27.5" H (46.5 cm x 45.5 cm x 69.8 cm)     
Without environmental control cover: 51 lbs (23.1 kg)     
With environmental control cover: 58 lbs (26.3 kg)     
CE and TUV marked. RoHS compliant. Models for In Vitro Diagnostic use are available.


Automated digital microscopy to 100x     
  • Automated image capture, automated image analysis, automate movie making     
  • Image-based and laser autofocus, auto LED intensity, auto exposure     
  • Image whole organisms to subcellular details     
    Four imaging modes, multiple imaging processes     
  • Brightfield, color brightfield, phase contrast and fluorescence     
  • Z-stacking and z-projection, montage and stitching     
  • Digital phase contrast     
    Gen5 3.0 Software tools     
  • Integrated image capture, analysis, annotation and movie making on a single platform     
  • Quick implementation, no extensive training required     
  • Gen5 Image+ and Gen5 Image Prime for expanded image analysis     
    Kinetic live cell assay support     
  • Environmental control cover contains temperature and gas     
  • Humidity chamber supports long term live cell kinetics     
  • Dual reagent injectors provide quick inject/image to observe fast reactions     
    Fully integrated and compact      
  • Thoughtful design offers quick installation and setup     
  • Tool-free connections for gas controller, injectors and environmental control cover     
  • Filter cubes and objectives easily accessible from the front panel
  • Photos

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