Advertisement

Macherey-Nagel NucleoSnap Plasmid Midi

Share:

NucleoSnap Plasmid Midi

  • New column design (snap-off column) for vacuum processing of large sample volumes     
  • Transfection-grade plasmid DNA for sensitive downstream applications     
  • Isolate up to 700 Μg plasmid DNA in only 35 minutes     
         
    Applications     
  • Typical downstream applications: transfection of common cell lines (lipofection, electroporation)     
  • Also suitable for standard molecular biology application such as cloning and sequencing
  • NucleoSnap Plasmid Midi
    Attributes
    Additional Specifications Technology:     
    Silica-membrane technology     
         
    Processing:     
    Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), centrifugation for elution     
         
    Format:     
    Snap-off column     
         
    Endotoxin level:     
    < 50 EU/µg DNA (EU = Endotoxin Units)     
         
    Sample material:     
    Up to 50 mL E. coli culture (OD600=5)     
         
    Lysate clarification:     
    NucleoSpin® Plasmid Filter Columns (2 min centrifugation)     
         
    Vector size:     
    < 25 kbp     
         
    Typical yield:     
    400–700 µg (50 mL culture, OD600=4, high-copy plasmid)     
         
    A260/A280:     
    1.8–1.9     
         
    Elution volume:     
    200–500 µL     
         
    Preparation time:     
    35 min/6 preps     
         
    Binding capacity:     
    1.5 mg

    Features

    Photos

    Manuals and Resources

    Note: We appreciate the support of individuals and companies making their documents and resources available to our community. If you find a document or resource that does not belong please let us know by emailing info@labwrench.com

    Picture of Macherey-Nagel NucleoSnap Plasmid Midi
    Since 1911 MACHEREY-NAGEL represents high quality, innovation, and reliability in biomolecular and chemical analysis. Friendly expert advice for our highly-valued...

    More about Macherey-Nagel

    Visit Macherey-Nagel

    Questions and Answers

    Recent Question Activity

    Be the first to Ask a Question

    Reviews

    Member Reviews

    There are no threads in Macherey-Nagel NucleoSnap Plasmid Midi Reviews at this time.
    Add a Review

    Macherey-Nagel NucleoSnap Plasmid Midi Articles

    Helpful Resources, Articles, and Information

    Product: Midi preparation of plasmid DNA with transfection-grade purity using vacuum Name and Manufacturer: NucleoSnap Plasmid Midi...

    See All Related Articles
    Advertisement