NucleoSnap Plasmid Midi
New column design (snap-off column) for vacuum processing of large sample volumes Transfection-grade plasmid DNA for sensitive downstream applications Isolate up to 700 Μg plasmid DNA in only 35 minutes
Applications Typical downstream applications: transfection of common cell lines (lipofection, electroporation) Also suitable for standard molecular biology application such as cloning and sequencing
NucleoSnap Plasmid Midi
Additional Specifications |
Technology: Silica-membrane technology Processing: Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), centrifugation for elution Format: Snap-off column Endotoxin level: < 50 EU/µg DNA (EU = Endotoxin Units) Sample material: Up to 50 mL E. coli culture (OD600=5) Lysate clarification: NucleoSpin® Plasmid Filter Columns (2 min centrifugation) Vector size: < 25 kbp Typical yield: 400–700 µg (50 mL culture, OD600=4, high-copy plasmid) A260/A280: 1.8–1.9 Elution volume: 200–500 µL Preparation time: 35 min/6 preps Binding capacity: 1.5 mg |
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