Macherey-Nagel NucleoSnap Plasmid Midi

NucleoSnap Plasmid Midi

New column design (snap-off column) for vacuum processing of large sample volumes

Transfection-grade plasmid DNA for sensitive downstream applications

Isolate up to 700 Μg plasmid DNA in only 35 minutes

Applications

Typical downstream applications: transfection of common cell lines (lipofection, electroporation)

Also suitable for standard molecular biology application such as cloning and sequencing

NucleoSnap Plasmid Midi

Attributes
Additional Specifications	Technology: Silica-membrane technology Processing: Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), centrifugation for elution Format: Snap-off column Endotoxin level: < 50 EU/µg DNA (EU = Endotoxin Units) Sample material: Up to 50 mL E. coli culture (OD600=5) Lysate clarification: NucleoSpin® Plasmid Filter Columns (2 min centrifugation) Vector size: < 25 kbp Typical yield: 400–700 µg (50 mL culture, OD600=4, high-copy plasmid) A260/A280: 1.8–1.9 Elution volume: 200–500 µL Preparation time: 35 min/6 preps Binding capacity: 1.5 mg

Additional Specifications

Technology:
Silica-membrane technology

Processing:
Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold), centrifugation for elution

Format:
Snap-off column

Endotoxin level:
< 50 EU/µg DNA (EU = Endotoxin Units)

Sample material:
Up to 50 mL E. coli culture (OD600=5)

Lysate clarification:
NucleoSpin® Plasmid Filter Columns (2 min centrifugation)

Vector size:
< 25 kbp

Typical yield:
400–700 µg (50 mL culture, OD600=4, high-copy plasmid)

A260/A280:
1.8–1.9

Elution volume:
200–500 µL

Preparation time:
35 min/6 preps

Binding capacity:
1.5 mg

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