Bio-Rad Laboratories, Inc. ChemiDoc™ MP Imaging System


ChemiDoc™ MP Imaging System

The ChemiDoc MP Imaging System is a full-feature instrument for imaging and analyzing gels and western blots. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, general gel documentation applications, and stain-free technology imaging needs.
ChemiDoc™ MP Imaging System
Depth 61 cm
Height 53 cm
Width 51 cm
Power Requirements 100–250 V
Weight 35 kg
Additional Specifications Smart Tray Technology™:     
Automatic recognition of application-specific tray and adjustment of imaging parameters and software options     
Precalibrated focus for any zoom setting or sample height     
Two user-defined modes (rapid or optimal auto-exposure) for all blot and gel imaging applications     
Image flat fielding:     
Dynamic; precalibrated and optimized for each application     
Touch-screen functionality:     
Multitouch capability (2 points) 12.1" display     
Maximum image area (W x H):     
21 x 16.8 cm     
Cooled CCD, 6 megapixels     
Dynamic range:     
>4 orders of magnitude     
Illumination sources:     
  • Trans-UV, 302 nm excitation     
  • Epi-white     
  • Trans-white (requires White Sample Tray)     
  • Trans-blue, 450?490 nm excitation (requires Blue Sample Tray)     
  • Epi-blue, 460?490 nm excitation     
  • Epi-green, 520?545 nm excitation     
  • Epi-red, 625?650 nm excitation     
  • Epi-far red, 650?675 nm excitation     
  • Epi-near IR, 755?777 nm excitation     
    Filter and application:     
  • 590/110 nm standard filter to perform protein and DNA gel and blot imaging     
  • Chemiluminescence filter     
  • 518–546 nm filter for green-emitting fluorophores and stains     
  • 577–613 nm filter for yellow/orange-emitting fluorophores and stains     
  • 675–725 nm for red-emitting fluorophores and stains     
  • 700–730 nm filter for far red–emitting fluorophores and stains     
  • 813–860 nm for near IR–emitting fluorophores and stains     
    Data output:     
    16-bit or 8-bit: SCN, TIFF, JPEG files     
    Operating temperature:     
    Operating humidity:     
    10–85% relative humidity (non-condensing)
  • Features

    Features and Benefits     
  • All-in-one flexible imaging — get precise, reproducible fluorescence, chemiluminescence, and colorimetric gel and blot detection, analysis and documentation in a single system     
  • Improve your imaging capabilities with the ChemiDoc MP — benefit from the advantages of using a wider range of fluorescent reporters (RGB, far red, and near infrared) including StarBright™ Fluorophores; see the fluorophore compatibility chart     
  • Multiplex fluorescent western blotting — detect up to three proteins simultaneously and eliminate the need for stripping and reprobing     
  • Ease of use — features include automatic selection of optimal light source by application, auto focus, auto exposure, and preview features ensure optimal images. Learn in minutes with on screen help     
  • Stain-free protein normalization — stain-free imaging permits the normalization of bands to total protein in both in gels and blots; eliminates the need for housekeeping proteins     
  • Sample Trays and Smart Tray Technology™ — three sample trays available to cover diverse imaging applications. Smart Tray Technology automatically recognizes the application-specific trays and adjusts imaging parameters and software options accordingly     
    For western blotting, the ChemiDoc™ MP V3 Western Workflow™ provides the ability to rapidly assess electrophoresis and blot transfer quality prior to blot processing, to perform total protein normalization and multiplexing using a wide rance of fluorophores.     
    Applications and Uses     
  • Electrophoresis — protein, including stain-free, and nucleic acid gel imaging and documentation     
  • Blotting — identify and quantify proteins, nucleic acids, protein-ligand interactions, etc. with fluorescence, chemiluminescence, or colorimetric detection     
    Included Components     
  • Internal computer with Image Lab™ Touch Software     
  • 12” touch screen display     
  • Image Lab Software to install on your computer     
  • Blot/UV/Stain-Free Sample Tray for Bio-Rad stain-free gels and blots, fluorescence multiplexing, chemiluminescence detection, ethidium bromide and SYPRO Ruby (12003028)     
  • Clarity™ (1705060) and Clarity Max™ (1705062) Western ECL Substrates; Precision Plus Protein™ All Blue Prestained (1610373) and Precision Plus Protein Unstained, Strep-tagged (1610363) Protein Standards     
    Mini and Midi ChemiDoc MP V3 Western Workflows also include:     
  • ChemiDoc MP Imaging System     
  • Mini-PROTEAN® Tetra Cell, 4-gel (1658004) or Criterion™ Cell (1656001) Electrophoresis Chamber     
  • PowerPac™ Basic Power Supply (1645050)     
  • Trans-Blot® Turbo™ Transfer System (1704150)     
  • 4–20% Mini-PROTEAN® TGX Stain-Free™ or 4–20% Criterion™ TGX Stain-Free™ Protein Gels     
  • Buffers, Nitrocellulose Transfer Packs, Immun-Blot® LF PVDF Membranes, and other consumables     
    Optional Components     
  • White Sample Tray for Coomassie Blue, copper, silver, and zinc stains (12003026)     
  • Blue Sample Tray for nucleic acid applications with GelGreen or any SYBR® Stain (12003027)     
  • Holder for ChemiDoc Sample Trays and UV Shield (1708377)     
  • Gel Alignment Templates for ChemiDoc MP and ChemiDoc Imaging Systems (12003915)     
  • UV Safety Shield for ChemiDoc MP and ChemiDoc Imaging Systems (12003914)
  • Photos

    Manuals and Resources

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