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Malvern Panalytical MicroCal PEAQ-DSC

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MicroCal PEAQ-DSC

The MicroCal PEAQ-DSC system provides highly sensitive, easy-to-use microcalorimetry that helps reduce the time and cost associated with stability testing and comparability analysis. The MicroCal PEAQ-DSC is a manual instrument with a cleaning device, which can be upgraded to an automated version upon request.     
     
Data generated by the MicroCal PEAQ-DSC provides critical guidance for biopharmaceutical development in protein engineering, (pre)formulation development, process development, manufacturing change control and biosimilarity and biocomparability studies. The integrated software streamlines workflows, facilitates non-subjective data analysis, performance qualification and compliance with 21 CFR Part 11 and Annex 11 regulations, all delivering high integrity data and driving productivity in biopharmaceutical research.     
     
Differential Scanning Calorimetry (DSC) is a powerful analytical tool for characterizing the thermal stability of proteins and other biomolecules. The technique measures the enthalpy (ΔH) and temperature (Tm) of thermally-induced structural transitions of molecules in solution. This information provides valuable insights into factors that stabilize or destabilize proteins, nucleic acids, micellar complexes and other macromolecular systems. The data are used to predict shelf-life of biomolecular products including biopharmaceuticals, to enable batch-to-batch and biosimilar versus innovator molecule comparisons, to develop purification strategies, to characterize and evaluate protein constructs, and to rank the affinities of ligands to their protein targets in small molecule drug discovery programs.     
     
The high-sensitivity MicroCal PEAQ-DSC system features automated data analysis to support the generation of high integrity thermal stability data and deliver compliance with regulatory requirements, whilst allowing easy integration into existing data handling and transfer systems. Unattended operation of the instrument after sample loading frees operator time. This complete system requires no additional accessories, reagents or consumables.

Models

Automated
Attributes

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Sample Volume 0 to 325 μL
Depth 68 cm
Height 70 cm
Width 101 cm
Power Requirements 100-240 V A/C, 50/60 Hz, 70 W (cell), 400 W (max, autosampler)
Additional Specifications Technology:
Differential Scanning Calorimetry

Measurement type:
Temperature midpoint (TM)
Enthalpy (ΔH)
Heat capacity change (ΔCp)

Cell:
Capillary

Cell material:
Tantalum

Cell volume:
130 ΜL

Sample capacity:
288 (6 × 96-well plates)

Typical sample concentration:
0.01 mg/mL – 10 mg/mL 1

Sample throughput:
?50 samples/day

Sample storage temperature range:
4°C - 40°C

Noise:
0.05 ΜCal/°C 2

Baseline repeatability:
1 ΜCal/°C 2

Response time:
5s 2

Repeatability:
<0.2 µCal/°C 3

Reproducibility:
<0.08°C St. Dev. T M and <2% RSD on ΔH 4

System reproducibility:
<0.1°C St. Dev. T M and <5% RSD on ΔH 4

Multiple feedback modes:
Yes (passive, high gain and low gain)

Temperature range:
2°C to 130°C 2, 5

Maximum scan rate:
240°C/h

Reverse scanning:
Yes

Pressure perturbation calorimetry (PPC):
N/A

Cleaning routines:
3 pre-programmed routines

Cleaning solvents:
Water and detergent used as standard

21 CFR part 11:
Yes, with PEAQ-Compliance software option

Network ready:
Yes, with email alert capability

Operating temperature (°C):
+10°C to +28°C

Storage temperature:
-20°C to +50°C

Humidity:
10% to 70%, non-condensing (10% to 90% for storage)

Ingress Protection (IP) rating:
IP21
Manual
Attributes

Edit Model

Sample Volume 0 to 250 μL
Depth 44 cm
Height 19 cm
Width 20 cm
Power Requirements 100-240 V A/C, 50/60 Hz, 70 W
Additional Specifications Technology:
Differential Scanning Calorimetry

Measurement type:
Temperature midpoint (TM)
Enthalpy (ΔH)
Heat capacity change (ΔCp)

Cell:
Capillary

Cell material:
Tantalum

Cell volume:
130 ΜL

Typical sample concentration:
0.01 mg/mL – 10 mg/mL 1

Sample throughput:
?6 samples analyzed/8h

Noise:
0.05 ΜCal/°C 2

Baseline repeatability:
1 ΜCal/°C 2

Response time:
5s 2

Repeatability:
<0.2 µCal/°C 3

Reproducibility:
<0.08°C St. Dev. T M and <2% RSD on ΔH 4

System reproducibility:
<0.1°C St. Dev. T M and <5% RSD on ΔH 4

Multiple feedback modes:
Yes (passive, high gain and low gain)

Temperature range:
2°C to 130°C 2, 5

Maximum scan rate:
240°C/h

Reverse scanning:
Yes

Pressure perturbation calorimetry (PPC):
N/A

Cleaning routines:
3 pre-programmed routines

Cleaning solvents:
Water and detergent (Contrad 90) used as standard

21 CFR part 11:
Yes, with PEAQ-Compliance software option

Network ready:
Yes, with email alert capability

Operating temperature (°C):
+10°C to +28°C

Storage temperature:
-20°C to +50°C

Humidity:
10% to 70%, non-condensing (10% to 90% for storage)

Ingress Protection (IP) rating:
IP21

Features

  • Gold standard stability-indicating technique requiring minimal assay development     
  • Direct and label-free measurement of biomolecular native-state stability in solution      
  • Measurement of very tight binding constants, up to 1020M-1     
  • Manual system with pipette and cleaning device     
         
         
    Powerful MicroCal PEAQ-DSC software minimizes typical data analysis time and includes:     
  • PEAQ-Compliance: The system is available with Malvern Access Controller (MAC) to limit access to user-defined SOPs and data analysis features. Data can be displayed using the Report Generator with user details and the ability to sign using electronic signatures to facilitate the integration of the workflow into your company’s quality system and aid compliance with 21 CFR Part 11 and Annex 11 regulations. This is provided as an optional accessory     
  • PEAQ-Performance: Automatically detects and validates system readiness for optimal performance     
  • PEAQ-Smart (including PEAQ-Finder): For SOP-based operation and data analysis. Provides new algorithms to detect even subtle peaks and shoulders, facilitating the automatic, non-subjective identification of multiple transitions, such as those seen in multi-domain proteins     
  • PEAQ-Compare: For the quantitative comparison of DSC traces for comparability studies, ideal for batch-to-batch and biosimilarity studies     
  • Network-ready: email updates sent during the analysis to keep you informed of assay progress
  • Photos

    Manuals and Resources

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    Instrument Documents

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