AMSbio CaMPARI Calcium Indicators


CaMPARI Calcium Indicators

CaMPARI, or Calcium Modulated Photoactivatable Ratiometric Integrator is a photoconvertible protein construct, enables imaging of integrated calcium activity of large populations of cells over a defined time window. Calcium indicators have found extensive use for imaging and measuring changes in Ca2+ levels associated with neural activity. 
CaMPARI is based on EosFP, a fluorescent protein whose emission changes from green to red upon irradiation with UV-light (~400 nm). By engineering libraries of EosFP variants, researchers were able to develop a protein that undergoes significantly faster green-to-red photoconversion (PC) only when calcium is present while PC light is applied. This permanent conversion provides the ability to record calcium activity for all areas illuminated by PC-light. The red fluorescence intensity correlates with calcium activity.


Key advantage of CaMPARI 
•Image total calcium activity during defined time windows (gated by photoconversion light). 
•Not restricted to the field of view of a microscope, as during real-time calcium imaging with e.g. GCaMP. 
•Enables higher-throughput calcium assays with cultured cells. 
•Calcium activity imaging across large cell populations and/or tissues. 
•Labeling of “active” cells within a tissue (such as brain) during stimulus or behavior in model organisms.  
•Tracing of neurons based on their calcium activity level.


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