Advertisement

Bio-Rad Laboratories, Inc. StarBright™ Blue 700 Fluorescent Secondary Antibodies

Share:

StarBright™ Blue 700 Fluorescent Secondary Antibodies

StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores. 
 
StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot. 
 
Schematic representation of a StarBright Blue 700 Label conjugated to an antibody. The light-absorbing monomer is excited at 470 nm and, via an efficient intramolecular transfer process, the energy is transmitted to a light-emitting monomer, which emits at 700 nm. An antibody is conjugated to linker monomers.

Features

Features and Benefits 
  • Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot 
  • Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes 
  • High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm) 
  • Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies 
     
    Applications and Uses 
    StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores as described in the table below. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
  • Photos

    Manuals and Resources

    Note: We appreciate the support of individuals and companies making their documents and resources available to our community. If you find a document or resource that does not belong please let us know by emailing info@labwrench.com

    Instrument Documents

    Picture of Bio-Rad Laboratories, Inc. StarBright™ Blue 700 Fluorescent Secondary Antibodies
    Bio-Rad, a global leader in supporting healthcare, provides the industry with useful products and services spanning the entire range of drug discovery...

    More about Bio-Rad Laboratories, Inc.

    Visit Bio-Rad Laboratories, Inc.

    Questions and Answers

    Recent Question Activity

    Be the first to Ask a Question

    Reviews

    Member Reviews

    There are no threads in Bio-Rad Laboratories, Inc. StarBright™ Blue 700 Fluorescent Secondary Antibodies Reviews at this time.
    Add a Review

    Bio-Rad Laboratories, Inc. StarBright™ Blue 700 Fluorescent Secondary Antibodies Articles

    Helpful Resources, Articles, and Information

    StarBright Blue 700 Secondary Antibodies Offer Improved Sensitivity among Fluorescent Dye Labeled Secondary Antibodies...

    See All Related Articles

    Page Navigation

    Advertisement