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Bio-Rad Laboratories, Inc. Aminex® Carbohydrate Analysis Columns

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Aminex® Carbohydrate Analysis Columns

Aminex HPLC columns are packed with a polymer-based matrix (polystyrene divinylbenzene). They separate compounds using the ion-moderated partition chromatography technique. Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms: 
 
  • In oligosaccharide separations, size exclusion is the primary mechanism. Low crosslinked resins allow carbohydrates to penetrate, and oligosaccharides separate by size 
     
  • For monosaccharide separations, ligand exchange is the primary mechanism and involves the binding of hydroxyl groups of the sugars with the fixed counterion of the resin. Ligand exchange is affected by the nature of the counterion (Pb2+, Ca2+, etc.) and by the spatial orientation of the carbohydrate's hydroxyl groups 
     
    Aminex columns allow the use of simple isocratic methods, eluting with water or dilute acid. There is minimal sample preparation, usually just filtering through a 0.45 µm filter with no derivatization necessary. Aminex media have a high pressure stability, wide pH stability, and high column efficiency and selectivity. 
     
    Aminex columns are an industry standard for the analysis of carbohydrates. Bio-Rad offers a complete line of carbohydrate analysis columns optimized to provide high selectivity for a specific carbohydrate or carbohydrate class. Research length (300 mm) columns provide high-resolution separations of complex carbohydrate mixtures in approximately 20 minutes, while the shorter columns provide efficient separations of specific carbohydrates in 3–5 minutes.
  • Models

    Aminex HPX-87C Column
    Attributes

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    Height 250 mm
    Width 4 mm
    Additional Specifications
  • Optimized for analyzing monosaccharides
  • This calcium-form column is the column of choice for most general sweetener analyses

    Also available in 300 mm x 7.8 mm
  • Aminex HPX-87P Column
    Attributes

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    Height 300 mm
    Width 7.8 mm
    Additional Specifications
  • Used in analyses of pentoses and hexoses in wood products 
     
  • Tailored for the separation of cellulose-derived monosaccharides

    Lead-Form
  • Fast Carb Analysis Column
    Attributes

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    Height 100 mm
    Width 7.8 mm
    Additional Specifications
  • Tailored for extremely fast separations of specific carbohydrates in samples where only certain components are of interest
  • Optimized for 5-minute analyses of sucrose, glucose, galactose, and fructose.
  • lead-form
  • Aminex HPX-87H Column
    Attributes

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    Height 300 mm
    Width 7.8 mm
    Additional Specifications
  • Hydrogen-form
  • Analysis of carbohydrates in solution with carboxylic acids, volatile fatty acids, short chain fatty acids, alcohols, ketones, and many neutral metabolic by-products.
  • Aminex HPX-87N Column
    Attributes

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    Depth 7.8 mm
    Height 300 mm
    Additional Specifications
  • Analysis of sugars in samples with high salt concentrations, such as beet sugars.
  • No need to desalt samples before analysis.
  • Sodium-form
  • Aminex HPX-87K Column
    Attributes

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    Height 300 mm
    Width 7.8 mm
    Additional Specifications
  • Potassium-form
  • Analysis of mono-, di-, and trisaccharides in samples such as corn syrup and brewing wort.
  • Aminex HPX-42A Column
    Attributes

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    Height 300 mm
    Width 7.8 mm
    Additional Specifications
  • Silver-form
  • Fast, high-resolution oligosaccharide analysis.
  • Separates oligosaccharides to D-11 in approximately 25 minutes.
  • Aminex HPX-42C Column
    Attributes

    Edit Model

    Height 300 mm
    Width 7.8 mm
    Additional Specifications
  • Calcium-form
  • Analysis of mono- and disaccharides in starch hydrolysates.
  • Excellent resolution of oligosaccharides as large as D-10.
  • In-Line Carb Deashing System
    Attributes

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    Additional Specifications
  • Protects the Aminex carbohydrate columns from contamination by removing all inorganic salts from the sample.
  • Consists of two Micro-Guard cartridges.
  • Features

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    Manuals and Resources

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