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Horizon EGFR Multiplex cfDNA

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EGFR Multiplex cfDNA

The EGFR Multiplex cfDNA Reference Standard Set is a cell line-derived, clinically relevant control that can be used to assess the performance of cfDNA assays that detect somatic resistance mutations in EGFR. 
 
With this product, you are able to:  
• Analyze the sensitivity and specificity of your assay  
• Gain certainty of the limit of detection and limit of quantification  
• Optimize and validate new EGFR-specific assays and routinely monitor their performance
EGFR Multiplex cfDNA
Attributes
Additional Specifications Technical Data 
Genes Covered EGFR  
Allelic Frequencies 5%, 1%, 0.1% and 0% (EGFR Multiplex wild type)  
Buffer Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0 
 
Product Information 
Fragment Size 160 bp  
Unit Size 350 ng per vial 
Concentration 20 ng/µl 
 
Presence confirmed in parental cell line EGFR Q787Q, EGFR L844L 
 
General Information 
Storage 4°C  
Expiry 12 months from the date of manufacture 
 
Quality Control 
Fragmentation Size D1000 DNA Screen Tape assay  
Allelic Frequency Droplet Digital™ PCR 
Quantification Qubit dsDNA BR Assay (post-fragmentation)

Features

High-throughput platforms such as PCR-based assays and Next Generation Sequencing (NGS) give laboratories much greater power when profiling cfDNA samples. However, small quantities of DNA in combination with complex variants at low allelic frequencies represents one of the major challenges users encounter when profiling cfDNA.  
 
Horizon Discovery has developed a comprehensive cfDNA Reference Standard range to support the development and continued validation of cfDNA assays. Our cfDNA products are derived from human cell lines and fragmented to an average size of 160 bp to closely resemble cfDNA extracted from human plasma.  
 
The EGFR Multiplex cfDNA Reference Standard is supplied at 5%, 1%, 0.1% and 0% (EGFR Multiplex wild type) allelic frequencies and covers ten EGFR variants implicated in the responsiveness to EGFR tyrosine kinase inhibitors (EGFR-TKIs) and anti-EGFR monoclonal antibodies. This product covers clinically relevant SNPs, insertions and deletions in EGFR and can be used to optimize, validate or routinely monitor assay performance.

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