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Advanced Analytical Inc. High Sensitivity Fragment Analysis Kits

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High Sensitivity Fragment Analysis Kits

A key biomacromolecule in the growing area of liquid biopsies, cfDNA could provide non-invasive and safer screening procedures to assess fetal health, detect the early stages of various cancers and rejection of transplants. Found in various bodily fluids including serum, plasma, saliva, cerebral spinal fluid (CSF), and urine, cfDNA can be isolated from individuals in both healthy and various disease states. In healthy individuals, cfDNA is actively secreted from cells as DNA is continually synthesized by cell turn over through apoptosis. cfDNA associated with disease states is released due to different types of cell death such as apoptosis, necrosis, autophagy, or mitotic catastrophe. Apoptosis activates endogenous endonucleases that cleave DNA in repeatable lengths. DNA is wrapped around nucleosomes like "beads on a string". The pattern of cfDNA cleavage is guided by the nucleosome positioning. Variations of 165 bp cfDNA lengths and multiples thereof are a result of the length of DNA wrapped around the nucleosome, the different DNA linker lengths between the nucleosomes, and degradation of the linkers between the nucleosomes.

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DNF-464 High Sensitivity Kit
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Additional Specifications The High Sensitivity Large Fragment 50 Kb Analysis Kit is used for the automated quality and quantity analysis of large DNA fragments and smears. The inclusion of a 48.5 Kb DNA fragment in the DNA ladder enables the sizing of DNA fragments and smears through 50 Kb. The enhanced sizing range of the kit directly relates to its versatility. It is employed equally well as a quality assurance step for downstream applications such as next-generation long read sequencing.  
  
 
Specifications 

Sample Volume Required: 2 µL 

Diluent Marker Volume Required: 22 µL 

DNA Sizing Range: 75 bp - 48,500 bp 

DNA Sizing Accuracy: ± 15% or better 

DNA Fragment Concentration Range: 5 pg/µL – 600 pg/µL input DNA (optimal concentration 500-600 pg/µL) 

DNA Smear Concentration Range: 50 pg/µL - 5 ng/µL input DNA (optimal concentration of 1 ng/µL) 

DNA Quantification Accuracy: ± 25 % 

DNA Quantification Precision: 20 % CV 

Maximum DNA Concentration: 600 pg/µL per fragment; 5 ng/µL per total sample 

Total Electrophoresis: Run Time 35 min (Ultra-Short Array, 22-47); 55 min (Short Array, 33-55)
DNF-493 High Sensitivity Kit
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Additional Specifications The DNF-493 High Sensitivity Large Fragment Analysis Kit (DNF-493 HS Large Fragment Kit) offers complete separation with accurate and reliable sizing and quantification of all three common cfDNA fragments. The sizing range of 75 bp - 20,000 bp permits the complete separation of fragmented DNA from the cfDNA fragments, allowing for accurate quantification of the total cfDNA sample. The DNF-493 HS Large Fragment Kit offers separation and visualization of any cfDNA fragments larger than the tri-nucleosome fragment. The DNF-493 HS Large Fragment Kit is a reliable option for the analysis of cfDNA in the presence of larger gDNA. 
 
Specifications
 
Sample Volume Required: 2 µL 

Diluent Marker Volume Required: 22 µL 

DNA Sizing Range: 50 bp – 20,000 bp 

DNA Sizing Accuracy: ± 15% or better 

DNA Fragment Concentration Range: 5 pg/µL – 0.5 ng/µL input DNA 

DNA Smear Concentration Range: 50 pg/µL - 5 ng/µL input DNA 

DNA Quantification Accuracy: ± 25% 

DNA Quantification Precision: 20% CV 

Maximum DNA Concentration: 0.5 ng/µL per fragment; 5 ng/µL total 

Total Electrophoresis Run Time: 50 minutes (Short Array); 80 minutes (Long Array)

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