Bio-Rad Laboratories, Inc. StarBright™ Blue 520


StarBright™ Blue 520

The StarBright Blue 520 Fluorophore is an ultra-sensitive polymer-based fluorophore with an excitation maximum at 488 nm and emission maximum at 520 nm. The StarBright Fluorophore is made up of multiple light-absorbing and -emitting monomers which provide an exceptionally bright signal compared to most traditional fluorophores. 
StarBright Blue 520 Conjugated Goat Anti-Mouse and Goat Anti-Rabbit Fluorescent Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single-target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. StarBright Blue 520 Fluorescent Secondary Antibodies can be used with far-red emission StarBright Blue 700 Antibodies as well as traditional fluorescent detection reagents like Alexa Fluor and DyLight Conjugated Antibodies. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or with hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.


Easy multiplexing — can be used with traditional fluorescent antibodies or with Bio-Rad's StarBright Blue 700 Fluorescent Secondary Antibodies to detect multiple proteins on the same blot 
High sensitivity — StarBright Blue 520 Fluorescent Secondary Antibodies (Exmax/Emmax = 488 nm/520 nm) have 2–4 fold higher sensitivity compared to traditional green emitters like Alexa Fluor 488 or DyLight 488 Antibodies 
Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes 
Low nonspecific binding — the StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies


Manuals and Resources

Note: We appreciate the support of individuals and companies making their documents and resources available to our community. If you find a document or resource that does not belong please let us know by emailing

Picture of Bio-Rad Laboratories, Inc. StarBright™ Blue 520
Bio-Rad, a global leader in supporting healthcare, provides the industry with useful products and services spanning the entire range of drug discovery...

More about Bio-Rad Laboratories, Inc.

Visit Bio-Rad Laboratories, Inc.

Questions and Answers

Recent Question Activity

Be the first to Ask a Question


Member Reviews

There are no threads in Bio-Rad Laboratories, Inc. StarBright™ Blue 520 Reviews at this time.
Add a Review

Bio-Rad Laboratories, Inc. StarBright™ Blue 520 Articles

Helpful Resources, Articles, and Information

 Bio-Rad Laboratories Inc. (NYSE: BIO and BIOb) today announced the launch of StarBright Blue 520 Fluorescent Secondary Antibodies, fluorescent...

See All Related Articles