Agilent Technologies 8453



The Agilent 8453 UV-Visible spectrophotometer uses a photodiode array (PDA) for simultaneous measurement of the complete ultra-violet to visible light spectrum in less than one second. The PDA technique brings exceptional reliability and repeatability. Using Agilent ChemStation software you get powerful data analysis and data archival to ensure compliance with good laboratory practices (GLP) and pharmacopoeia requirements.
Light Source Easy to exchange prealigned deuterium and tungsten lamps
Photodetector Diode Array
Photometric Noise <0.0002 A sixty 0.5-s scans at 0 A, 500 nm, rms
Scanning Speed 1.5 second, full range
Stray Light <0.03% at 340 nm (NaNO2, ASTM), <0.05% at 220 nm (Nal, ASTM), <1% at 198 nm (KCl, EP)
Wavelength Range 190 to 1100 nm
Wavelength Resolution 1.6 nm
Depth 22 in
Height 7.3 in
Width 13.5 in
Power Requirements 90–264 V AC 47–63 Hz 70 VA typical
Weight 36.3 lbs
Additional Specifications Optical Specifications
Slit width: 1 nm
ratio abs. at 269 nm/ 266 nm
Wavelength accuracy (NIST 2034)*:
<±0.5 nm at 486.0 nm
<±0.2 nm at 656.1 nm
Photometric accuracy: <±0.005 A, <±0.01 A at 1 A (NIST 930e) potassium dichromate, EP method
Photometric stability: <0.001 A/h at 0 A, 340 nm, after 1 hour warm-up, measured over 1 hour, every 5 seconds, Constant ambient temperature
Baseline flatness: <0.001 A; 0.5-second blank, 0.5-second scan, rms
Humidity: <95% at 25 to 40 °C (77 to 104 °F)

* The methods used are application relevant, for example, the wavelength accuracy specification is given for the complete wavelength range and not just for a single wavelength.

Computer Requirements (for ChemStation software)
Processor: 1 GHz 32-bit (x86) or 64-bit (x64)
RAM: At least 1 GB
Peripherals: CD-ROM disk drive
Hard disk drive: At least 40 GB of free capacity
Operating system: Microsoft Windows XP or VISTA
Monitor: Super VGA or Ultra VGA 17 inch color with resolution at least 800x600 pixel
Video: 256 colors and 1 MB video RAM, small fonts are recommended
Interface: LAN


  • Fast results – UV-Vis spectral acquisition and analysis in less than 1 second
  • Excellent reproducibility – stable optics, low noise electronics, no moving parts
  • Simple operation – task-oriented and application software, direct access to cells and cell holder
  • Effortless servicing – pre-aligned deuterium and tungsten lamps exchanged easily
  • Good laboratory practices (GLP) – self-test and revisions built into the software/firmware
  • Pharmaceutical compliance – regulated software for European and US pharmacopoeia
  • Flexible accessories – autosamplers, thermostatted cell holders, multicell transports, sipper systems


8453 UV-Visible Spectrophotometer

8453 UV-Visible Spectrophotometer

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power fail

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Error message: CPtrg: Powerfail 8453

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Lan not detected

Got the new lan card G1369, for the 8543 spectrograph. But, the computer does not even recognise the lan connection. Same problem with the damaged card.   Please help

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See it in action

UV-Visible Spectroscopy Video

View this video on a separate website3

View a demonstration on how easy it is to make a complete spectral measurement using the Agilent 8453 UV-VIS Spectrophotometer and Agilent UV-VIS...

8453 UV-Visible Spectrophotometer

View this video on a separate website3

View a demonstration on how easy it is to make a complete spectral measurement using the Agilent 8453 UV-VIS Spectrophotometer and Agilent UV-VIS...