New Brunswick Scientific BioFlo 4500


BioFlo 4500

The BioFlo 4500 Has Been Replaced By The BioFlo 415

The BioFlo 4500 is an industrial fermentor/bioreactor with the power, versatility and advanced controls for microbial, insect and mammalian cell culture,in applications ranging from research through small-scale production. A wide array of options enable configuration to your individual process requirements, without the high cost and delays normally associated with purchasing a custom fermentor. The system’s most powerful feature is its advanced control capability, driven by NBS ML-6100™ multi-loop controller with touch-screen interface. With the ML-6100, on-screen entry of process control loops, sterilization cycles and calibration routines is remarkably intuitive, making the BioFlo 4500 exceptionally easy to operate. Almost any auxiliary process device, from sensors to biochemical analyzers, can be integrated with the fermentor without need for additional hardware, software or a service technician. In fact, every facet of this compact system has been designed with efficiency in mind. An open-frame design provides easy access to all piping, valves, regulators and filters; and assignable, peristaltic pumps with Easy-Load® pump heads speed tube loading. The system is fully cGMP compliant. For process flexibility and operating simplicity the BioFlo 4500 has no equal.

Models Include:
BioFlo 4500 20L
BioFlo 4500 30L


  • Multiple options allow for process-related configuration
  • Available with an embedded or external ML-6100 controller, or customer-specified SCADA system. The ML-6100 provides an intuitive user interface for fermentation operation and system configuration
  • For scale-up studies, the ML-6100 allows emulation of much larger bioreactors through programmable heating, cooling, and pre- and post-sterilization protocols
  • Capable of rapid temperature shifts in growth phase, to allow for heat induction (1°C/minute in range of 30°C to 45°C); with slower temperature rises also programmable
  • High oxygen transfer rates (OTR) of 350 mM O2/L/Hr minimum, attainable
  • Optional 2-gas, 3-gas and 4-gas mixers available for oxygen enrichment and cell culture applications
  • Capable of remote control, data logging and process programming using optional AFS-BioCommand® software
  • Only NBS has an in-house ASME-accredited vessel shop, microbiology and cell culture labs, software and instrumentation development group, and integrated cGMP
    bioprocess area to provide added customer support
  • Set-up and start-up assistance provided, with optional validation and training packages available. Process development and scale-up assistance can also be provided


BioFlo 4500

BioFlo 4500

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hard drive crashed

The Samsung SV0643A hard drive in my BioFlo 4500 is not booting.  I am going to try to recover the drive, however I would like any/all help in locating the software (NBS Local Controller Interface V 1.20) and/or an image of the drive, or a...

leak in mechanical seal rotor assembly

I see a steam/water leak from the vessel top mechanical seal that lets the impeller shaft through. Can you help me fix it?


we are facing a problem while pressurerise the vessel

See all 11 New Brunswick Scientific BioFlo 4500 Questions and Answers There are 11 Threads


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