HORIBA Horiba Duetta Fluorescence and Absorbance Spectrometer


Horiba Duetta Fluorescence and Absorbance Spectrometer

A Superior Spectrometer Concept   
The Future of Fluorescence   
  • Two-in-one Fluorescence and Absorbance Spectrometer   
  • UV-Vis-NIR Fluorescence Detection Wavelength Range from 250 to 1,100 nm   
  • Full 3-D Fluorescence EEM Acquisition in Less Than One Second   
  • Best in Class Fluorescence Sensitivity Specification of 6,000:1 RMS for Water Raman   
  • Automatic Correction for Primary and Secondary Inner Filter Effects (IFE)   
  • High Fidelity Molecular Fingerprinting with Unique A-TEEM™ (Absorbance-TransmittanceExcitation EmissionMatrix) Technology   
  • Millisecond CCD Detection of Entire Fluorescence Spectrum   
Fluorescence and Absorbance in one Spectrometer!   
Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.   
Fluorescence in the Blink of an Eye!   
Duetta’s ultra-fast, state-of-the-art CCD technology allows it to outperform, by orders of magnitude, any competitor using PMTs. It’s the fastest bench-top fluorometer in theworld,and the only fluorometer that can acquire a full spectrum in less than 100 milliseconds! This state-of-the-art CCD technology also extends NIR wavelength detection to 1,100 nm, well beyond the limit of a standard PMT fluorometer.   
Highest Fluorescence Sensitivity in its Class   
Having the highest level of fluorescence sensitivity means that Duetta’s optimized design lets you measure lower concentrations ofsample, and providesbetterquality of data.   
EzSpec™ Touch-Screen Software!   
Duetta debuts HORIBA’s new generation software interface with touch-screen utility, and a selection of Apps for routine analysis.   
A New Spectrometer Design   
Fluorescence and Absorbance   
Duetta’s two specialized sample detectors simultaneously collect fluorescence at right angles to the excitation light, and absorbance straight through the sample.   
Absolute Fluorescence Spectra with Real Time Inner Filter Effect (IFE) Correction   
IFE limits the linearity of the fluorescence signal at higher sample concentrations due to primary and secondary re-absorption of fluorescence. Duetta’s unique design applies real time IFE correction  
to obtain absolute fluorescence spectra at higher sample  
CCDs Collect the Entire Emission Spectrum   
Ergonomic, Hands Free, Sample Access   
Duetta’s CCD detection takes a snapshot of the entire fluorescence emission spectrum from 250 to 1,100 nm, so, not only is it much faster, but it also gives much more information than the competition.   
Extended NIR Wavelength Range up to 1100 nm   
Near Infrared applications are growing rapidly, and Duetta is ready to meet the challenge with fluorescence detection from 250 to 1,100 nm, well beyond the reach of standard PMTs.   
Emission from Neodymium Glass  
NIR detection comparing Duetta with PMT.   
Easy Lamp Cartridge Replacement Lamp   
Lamp cartridges are pre-aligned at the factory, guaranteeing perfect alignment upon snap-in replacement. No tools or service are required.   
Smart Sample Accessory Trays   
Switching from one type of sample holder to another could not be easier.   
Without any tools or cables, Duetta’s spill-proof sample accessory trays are identified by EzSpec software,  
enable electrical power for the appropriate module (e.g. 4-position Peltier holder), and read an enabled  
sensor (e.g. temperature), if available.   
Fully Automated Order Sorting Filters   
Duetta includes excitation and emission cut off filters for automated elimination of second  
order grating effects, which otherwise interfere with the fluorescence signal of interest.   
Excitation Emission Matrix (EEM) acquired in 1 second, shows no second order scattering.  



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