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Molecular Devices pCLAMP 10

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pCLAMP 10

The pCLAMP 10 software suite fulfills many different experimental needs, such as synchronized stimulation, event detection, and online analysis. It is the most widely-used data acquisition and analysis program for the control and recording of voltage-clamp, current-clamp, and patch-clamp experiments. Three separate programs are included: Clampex 10, AxoScope 10, Clampfit 10.

Clampex 10 expands the range and quality of your data acquisition experiments. These improvements should prove useful for a wide variety of applications, allowing more flexibility in your experimental protocols.
pCLAMP 10
Attributes
Additional Specifications System Requirements
Operating system: US Windows® XP Pro SP2 or 2000 SP4
CPU: 1.2 GHz Pentium (2.4 GHz recommended)
Drives: 500 MB HD space DVD/CD-ROM
Display: 1024 x 768 resolution
Ports:
USB 1 (for security key)
USB 1.1 (for MiniDigi 1)
USB 2.0 (for Digidata 1440A)
Slots: PCI (for Digidata 1322A)

Bundled Hardware
Digitizer type: MiniDigi 1
Channels: 2
Channel type: Analog input
Input range: ±10 V
Resolution: 16 bits
Sampling rates: 1 Hz– 1 kHz
Filter types: Analog, min/max
Control software: AxoScope 10
Communication: USB 1.1

Features

  • Membrane Test calculates Ra and Cm per sweep during a recording
  • Membrane and Seal Tests combined into a single resizable window
  • Support for four stimulus waveforms (with the Digidata 1440A)
  • Control of eight digital outputs per epoch during a sweep
  • Control of split-clock sampling per epoch during a sweep
  • Leak subtraction automatically saves both raw and corrected traces
  • All protocol durations are entered in time units
  • Support of the new Digidata 1440A digitizer
  • Support of the new Axoclamp 900A amplifier
  • New ABF 2.0 file format

Existing functionality includes the ability to automate the execution of protocols, a variety of recording modes from continuous to waveform stimulation, online filtering and leak subtraction, and online statistics/graphs.

AxoScope 10 provides a convenient way to produce background recordings with the included MiniDigi 2-channel digitizer*. You can monitor cells during intra-sweep periods, or create an overview of the entire day's activities including voice tags

Clampfit 10 is a powerful solution for analyzing, graphing and layout of all of your Clampex and AxoScope data. Clampfit includes an extensive array of filtering and fitting routines. Functionality includes I-V graphs, power spectrums, and special "linked data views" for threshold (AP), template (minis) and single-channel modes of event detection and analysis.

Applications
pCLAMP 10 is suitable for a wide variety of applications, ranging from electrophysiology to general physiology, from individual cells to networked cells, and from low-noise patch clamping to high-speed sodium channels.

Uses:
  • Action Potentials (APs)
  • Current-clamp
  • Electromyography (EMG)
  • Electrooculography (EOG)
  • Excitatory Post-Synaptic Currents (EPSCs)
  • Excitatory Post-Synaptic Potentials (EPSPs)
  • Inhibitory Post-Synaptic Currents (IPSCs)
  • Inhibitory Post-Synaptic Potentials (IPSPs)
  • Long-Term Depression (LTD)
  • Long-Term Potentiation (LTP)
  • Miniature excitatory potentials (Minis)
  • Ratio dyes (with PMTs)
  • Single-channels
  • Slices
  • Spike trains
  • Synaptic networks
  • Voltage-clamp
  • Whole-cell

Photos

Single-Channel Analysis

Single-Channel Analysis

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Questions and Answers

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truncate the .abf gap-free record

Hi I am struggling to find an option to truncate my long gap-free record into one smaller to safe only the region of interest and save the disk space ... how to truncate the length of record in general in postprocessing? thanks!

problem in saving dat files in atf

I would like to save my dat files as atf  by using the pclamp10 option because I want to perfom other analyses. Actually I could do it until the files were aquired to a certain sample rates. Since I augmented the sample rates always using the...

weighted decay and charge transfer calculation with pclamp

Hi,I would like to know how to calculate the weighted decay and charge transfer of IPSCs using pclamp.                        ...

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