Setting up and using the Accuri C6 Flow Cytometer in a controlled environment glovebox.
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| Additional Specifications | Excitation: 488nm (rated at 20,000hr life) 640nm (rated at 20,000hr life) Laser profile: 15 x 75 microns Scatter detection: Forward (0 degrees, +/-15) Side (90 degrees, +/-15) Emission detection: 4 colors, user swappable optical filters Standard set installed: - FL1 530 / 30nm (FITC/GFP) - FL2 585 / 40nm (PE/PI) - FL3 >670nm (PE-Cy5, PE-Cy5.5, PerCP-Cy5.5,PE-Cy5, PE-Cy7) - FL4 675 / 25nm (APC) Optical alignment: Fixed alignment, no maintenance required Flow cell: 200 micron ID quartz capillary Minimum particle size: 1 µm Minimum sample size: 30 µl Nominal flow rate - variable: 10 to 100 µl/min ; CFlow Plus only Fixed Flow Rates & Core Sizes: Slow - (Flow = 14uL/min ; Core = 10 um) Medium - (Flow = 35uL/mim ; Core = 16 um) Fast - (Flow = 66 uL/min ; Core = 22 um) Recommended Sheath Fluid: 0.2 um filtered DI water Core diameter: User selectable from 5 to 40 microns, CFlow Plus Only Florescence sensitivity: <750 MESF FITC Scatter resolution: Resolves human peripheral blood erythrocytes, lymphocytes, monocytes & granulocytes Fluorescence linearity: 2+/-0.05% for chick erythrocyte nuclei (CEN) Fluorescence precision: <3% CV for CEN Speed: 10,000 events/second maximum Absolute counts: Yes; CFlow Plus only Warm-up time: Less than 5 minutes Power: 70 Watts maximum, 100-240 VAC, 50/60 Hz Heat output: 240 Btu/hour maximum output Operating ranges: 15-30°C; <80% relative humidity Instrument size: 11"H x 14.75"W x 16.5"D (27.9 x 37.4 x 41.9 cm) Foot print with fluid tanks: 11"H x 21.5"W x 16.5"D (27.9 x 54.6 x 41.9 cm) Weight: 30 lbs (13.6 kg) Fluid tank capacity: 1L Sheath Fluid, 1L Waste 250mL Cleaner, 250mL Decontamination Signal processing: 24-bit A/D conversion Computer: 19" (48.26 cm) flat-panel monitor, 4GB memory Computer interface: USB 2.0 |
C6 System
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Setting up and using the Accuri C6 Flow Cytometer in a controlled environment glovebox.
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