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BioTek ELx405

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ELx405

To meet the ever growing demand for automated washing of microsphere based assays, the industry leading ELx405 Microplate Washer is now available with modules for both vacuum filtration and biomagnetic separation. BioTek's latest ELx405 enhancements offer full plate washing of polystyrene and magnetic microspheres used in a growing number of multiplex assays and bead-based ELISAs, such as those developed on the Luminex xMAP® technology platform. An available vacuum filtration feature automates the wash steps of filter bottom plates with four adjustable vacuum levels for optical performance with filter pore sizes ranging from 0.45 µm to 1.2 µm. Developed with leaders in genotyping, gene expression and protein assays, the ELx405's biomagnetic separation incorporates high-energy neodymium iron boron magnets for rapid separation of micrometer and nanometer beads with superior retention.

The ELx405 HT is designed specifically to rapidly wash 384-well microplates and includes BioTek's patented Dual-Action™ manifold to ensure optimal performance and easy maintenance.

The ELx405 Select is the market leader in 384-/96-well washing. The Select also has our patented Dual-Action™ manifold, providing independent control of both dispense and aspirate manifolds for precise overfill washing and overflow protection in both 384- and 96-well microplates. This unique design washes both 384- and 96-well plates without the need for any hardware changes. With its angled dispense tubes, full control of dispense and aspiration rates and unique X, Y, Z positioning, the ELx405 Select is ideal for the most demanding wash requirements. The ELx405 Select CW is specially designed to accommodate loosely-adherent cell lines.

For those applications that use only 96-well microplates, the standard ELx405 Microplate Washer is an ideal choice for performance and value! The ELx405 is the most precise and reliable washer available. Many unique features lower background absorbance values and increase the signal-to-noise ratio of typical ELISA assays.

The ELx405's patent pending Ultrasonic Advantage™ offers effortless washer manifold maintenance. Scientists regard unexpected clogging of manifold tubes to be the largest contributor to assay failure. Cleaning these tubes is a time consuming and labor intensive process. BioTek's Ultrasonic Advantage™ incorporates the power of ultrasonic cleaning to make the ELx405 the first self-maintaining microplate washer. BioTek's Liquid Handling Control Software provides full PC control of the ELx405 in a familiar Microsoft Windows® environment, while its intuitive StepWise™ protocol creation makes LHC Software easy to use even for novice users.

A total solution, the ELx405 offers 384- and 96-well plate washing for multiplex bead assays, ELISAs and cellular assays along with filtration-to-waste processes... all together in a single, robot-compatible design.
ELx405
Attributes
Depth 17 in
Height 10 in
Width 14 in
Power Requirements Compatible with 100 to 240 V~ ± 10% @ 50-60 Hz
Weight 30 lbs
Additional Specifications Assays
Magnetic bead, polystyrene bead (model dependent)
  • Multiplex assays
  • Bead-based ELISA

ELISA
Cell-based assays (model dependent)
Filtration-to-waste processes (model dependent)
Separation: Biomagnetic separation, vacuum filtration (model dependent)
Microplate Types:
384- and 96-well (model dependent)
Standard height and low profile
Solid and filter bottom (model dependent)
  • Filter pore sizes 0.45 µM to 1.2 µM

Shaking: Programmable in minutes and seconds, up to 60 minutes
Intensities – slow, medium, fast or variable
Soak Time: Programmable in minutes and seconds, up to 60 minutes
Magnet:
High strength 96- and 384-well formats
Choice of two designs, custom for BioTek microplate washers:
  • Flat
  • Flat-bottom well - beads pulled to band across well bottom
  • Round-bottom well - beads pulled to button at well bottom
  • Ring - beads pulled to 4-zone ring at well bottom

Incorporated into carrier, removable for non-magnetic washing
Safety/Convenience/Maintenace:
Waste and vacuum sensing, fluid and flow detection
Aerosol cover
Adjustment utility for plate positioning
Overflow protection
Pre-programmed maintenance routines
Automation: BioStack™ Microplate Stacker - up to 50 plates (optional)
  • Except filter bottom plates

BioStack Twister II Microplate Handler - up to 320 plates (optional)
Onboard Software:
2 x 24 character LCD display
25 alphanumeric soft keys
Create, edit or run multiple protocols
PC Software: Liquid Handling Control™, for PC wash protocol programming and execution (optional)
ELx405 Interface Software, for use with LHC or robotic system integration (optional)
Operating Temperature: 15°C to 30°C (59°F to 86°F)
Humidity: 10% to 85%, non-condensing

Washing
Manifold Type:
384-/96-well washing:
Dual-Action™ 96-tube (8 X 12) manifold
384-well washing:
Dual-Action™ 192-tube (16 x 12) manifold
96-well washing:
96-tube (8 X 12) manifold
Washing Speed:
Solid bottom plates
1 asp. / disp. cycle, 400 µL/well, 384 wells, 192-tube manifold, final aspirate: < 20 seconds
3 asp. / disp. cycles, 100 µL/well, 384 wells, 96-tube manifold, final aspirate: < 80 seconds
3 asp. / disp. cycles, 300 µL/well, 96 wells, 96-tube manifold, final aspirate: < 30 seconds
Filter bottom plates:
Variable, based on wash parameters
Fluid Delivery: Internal positive displacement pump
Vacuum Filtration: Selectable vacuum levels:
0.45 µM 96-well plate:
Lowest: -111 mmHg
Low: -200 mmHg
Medium: -405 mmHg
High: -561 mmHg
0.45 µM 384-well plate:
Lowest: -108 mmHg
Low: -196 mmHg
Medium: -371 mmHg
High: -563 mmHg
1.2 µM 96-well plate:
Lowest: -117 mmHg
Low: -203 mmHg
Medium: -409 mmHg
High: -550 mmHg
1.2 µM 384-well plate:
Lowest: -117 mmHg
Low: -198 mmHg
Medium: -387 mmHg
High: -450 mmHg
Vacuum filtration time range:
5 to 999 seconds
Volume Range: 25 - 3000 µL/well (model dependent)
Selectable in 1 µL increments
Buffer Selection: Automatic switching for up to 4 wash buffers (model dependent)
Flow Rates: 1 - 11
Includes low flow, cell wash rates (model dependent)
Wash Cycles: 1 - 10
Dispense Precision: = 3% CV (model dependent)
Residual Volume:
Solid Bottom Plates
= 2 µL/well
Filter Bottom Plates
96-well:
Average increase weight of plate = 1.2 g after dispensing 300 µL to 0.45 µm plate, filtration 30 seconds, low vacuum, blotted
384-well:
Average increase weight of plate = 4.0 g after dispensing 80 µL to 1.2 µm plate, filtration 10 seconds, low vacuum, blotted
Sterilization: Chemical:
70% ethyl or isopropyl alcohol and 0.5% sodium hypochlorite solution
Ultrasonic Cleaning: Built-in ultrasonic bath for protein and salt crystal buildup
Programmable in hours and minutes, up to 60 minutes (model dependent)
Supply Bottle Volume: 4 L or 10 L (optional)
Regulatory: All BioTek microplate instrumentation is CE and ETL marked.

Features

Hardware:
  • Accommodates 384- and 96-well microplates
  • Polystyrene and magnetic bead assays along with standard ELISAs
  • Patented Dual-Action™ manifold on the ELx405™ HT and ELx405™ Select provides independent control of the aspirate and dispense manifolds for precise overfill washing and overflow protection
  • Internal positive displacement pump eliminates the use of troublesome pressure delivery systems and allows the use of any size dispense reservoir
  • Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates the need for blank plates when used in automated robotic systems
  • Fully variable dispense volumes, flow, and aspiration rates provide total control of the washing cycles from a gentle cell-washing application to vigorous wash for high-binding assays
  • Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
  • Optional external valve module connects four reagent bottles with automatic switching between reagents
  • BioStack compatible for automated washing of up to 50 standard height microplates

Software:
  • 75 programs
  • Link up to 10 programs (if valve module is attached, links multiple buffers automatically)
  • Pre/AutoPrime
  • 10 cycles/wash
  • Crosswise aspiration
  • Fully programmable:
    - dispense height
    - aspiration height
    - aspiration delay
    - soak
    - shake
    - aspiration rate
    - horizontal aspirate and dispense position
  • Photos

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    Ultrasonic Advantage

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    Automated Washer Manifold Maintenance