| Attributes | |
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| Particle Size | 3 µm |
| pH Range | 2 to 8.5 |
| Additional Specifications | Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 Μm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle. Agilent Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations. Column Phase Size Exclusion Packing Spherical, high purity, porous silica with a hydrophilic polymeric coating Pore Structure 100Å, 150Å, 300Å Column Exclusion Limits (in Daltons) 100Å MW range: 100 - 100,000 150Å MW range: 500 - 150,000 300Å MW range: 5,000 - 1,250,000 Operating Temperature Limit Recommended range: 10-30°C, maximum: 80°C Operating Pressure Limit Recommended operating pressure: 137 bar (2,000 psi) Maximum pressure: 240 bar (3,500 psi) Mobile Phase Compatability Recommended: 150 mM phosphate buffer, pH 7.0; other aqueous buffers with high and low salt can be used Working Flow Rate 0.1 - 1.25 mL/min for 7.8 mm ID columns 0.1 - 0.4 mL/min for 4.6 mm ID columns |
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last edited by: LabWrenchData2 |
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| Particle Size | 5 µm |
| pH Range | 2 to 8.5 |
| Additional Specifications | Agilent Bio SEC-5 HPLC columns are packed with 5 Μm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution. Agilent Bio SEC-5 HPLC columns are available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å. Column Phase Size Exclusion Packing Spherical, high purity, porous silica with a hydrophilic polymeric coating Pore Structure 100Å, 150Å, 300Å, 500Å, 1000Å, 2000Å Column Exclusion Limits (in Daltons) 100Å MW range: 100 - 100,000 150Å MW range: 500 - 150,000 300Å MW range: 5,000 - 1,250,000 500Å MW range: 15,000 - 5,000,000 1000Å MW range: 50,000 - 7,500,000 2000Å MW range: >10,000,000 Operating Temperature Limit Recommended range: 10-30°C, maximum: 80°C Operating Pressure Limit Recommended operating pressure: 137 bar (2,000 psi) Maximum pressure: 240 bar (3,500 psi) Mobile Phase Compatability Recommended: 150 mM phosphate buffer, pH 7.0; other aqueous buffers with high and low salt can be used Working Flow Rate 0.1 - 1.25 mL/min for 7.8 mm ID columns 0.1 - 0.4 mL/min for 4.6 mm ID columns |
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last edited by: LabWrenchData2 |
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| pH Range | 2 to 12 |
| Additional Specifications | The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies. Bio MAb columns are available in 1.7 Μm, 3 Μm, 5Μm, and 10 Μm sizes, providing higher resolution with the smaller particles. The Bio MAb column features a highly uniform, densely packed, weak cation exchange layer. This layer of the Bio MAb column is chemically bonded to the hydrophilic, polymeric coating of the Bio MAb column. Column Phase Weak Cation Exchange (carboxylate) Packing Non-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating and bonded with a uniform, weak cation exchange layer Particle Size 1.7, 3, 5, and 10 Μm Pore Structure Non-porous Operating Temperature Limit 80°C Column Hardware Operating Pressure Limit 600 bar (8,700 psi) for stainless steel column hardware 400 bar (5,800 psi) for PEEK column hardware Particle operating pressure limit 275 bar (4,000 psi) for 10 Μm particles 413 bar (6,000 psi) for 5 Μm particles 551 bar (8,000 psi) for 3 Μm particles 689 bar (10,000 psi) for 1.7 Μm particles Mobile Phase Compatability Compatible with aqueous solution buffers, acetonitrile/acetone/methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate Working Flow Rate Typical 0.1-1.0 mL/min for a 4.6 mm ID column |
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last edited by: LabWrenchData2 |
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| pH Range | 2 to 12 |
| Additional Specifications | Agilent Bio Ion Exchange HPLC columns are packed with polymeric, nonporous, ion exchange particles. Bio Ion Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation exchange (SCX), weak cation exchange (WCX), strong anion exchange (SAX) and weak anion exchange (WAX) phases. All Bio Ion Exchange phases are available in 1.7, 3, 5 and 10 Μm non-porous particles sizes. Column Phase SCX (Strong cation exchange) – SO3H WCX (Weak cation exchange) – COOH SAX (Strong anion exchange) – N(CH3)3 WAX (Weak anion exchange) – N(C2H5)2 PackingNon-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating and bonded with a uniform, ion exchange layer Particle Size 1.7, 3, 5, and 10 Μm Pore Structure Non-porous Dynamic Binding Capacity SCX NP3: 53 mg/mL, SCX NP5: 38 mg/mL, SCX NP10: 20 mg/mL WCX NP3: 19 mg/mL, WCX NP5: 15 mg/mL, WCX NP10: 10 mg/mL SAX NP3: 35 mg/mL, SAX NP5: 28 mg/mL, SAX NP5: 17 mg/mL WAX NP3: 26 mg/mL, WAX NP5: 18 mg/mL, WAX NP3: 12 mg/mL Operating Temperature Limit 80°C Column Hardware Operating Pressure Limit 600 bar (8,700 psi) for stainless steel column hardware 400 bar (5,800 psi) for PEEK column hardware Particle Operating Pressure Limit 275 bar (4,000 psi) for 10 Μm particles 413 bar (6,000 psi) for 5 Μm particles 551 bar (8,000 psi) for 3 Μm particles 689 bar (10,000 psi) for 1.7 Μm particles Mobile Phase Compatability Compatible with aqueous solution buffers, acetonitrile/acetone/methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate. Working Flow Rate Typical 0.1-1.0 mL/min for a 4.6 mm ID column |
Agilent BioHPLC Columns
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