Agilent Technologies BioHPLC Columns

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BioHPLC Columns

Agilent Size Exclusion and Ion Exchange BioHPLC columns, featuring four new column families that enable highly reproducible and high resolution analytical separations of monoclonal antibodies, proteins, peptides and other bio-molecules.

Models

Agilent Bio SEC-3 HPLC Columns
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Particle Size 3 µm
pH Range 2 to 8.5
Additional Specifications Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 Μm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle. Agilent Bio SEC-3 HPLC columns are available in 100Å, 150Å and 300Å pore sizes to accommodate most peptide and protein size exclusion separations. 
 
Column Phase Size Exclusion  
 
Packing Spherical, high purity, porous silica with a hydrophilic polymeric coating  
 
Pore Structure 100Å, 150Å, 300Å  
 
Column Exclusion Limits (in Daltons)  
100Å MW range: 100 - 100,000 
150Å MW range: 500 - 150,000 
300Å MW range: 5,000 - 1,250,000  
 
Operating Temperature Limit  
Recommended range: 10-30°C, maximum: 80°C  
 
Operating Pressure Limit  
Recommended operating pressure: 137 bar (2,000 psi) 
Maximum pressure: 240 bar (3,500 psi)  
 
Mobile Phase Compatability  
Recommended: 150 mM phosphate buffer, pH 7.0; other aqueous buffers with high and low salt can be used  
 
Working Flow Rate  
0.1 - 1.25 mL/min for 7.8 mm ID columns 
0.1 - 0.4 mL/min for 4.6 mm ID columns  
Agilent Bio SEC-5 HPLC Columns
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Particle Size 5 µm
pH Range 2 to 8.5
Additional Specifications Agilent Bio SEC-5 HPLC columns are packed with 5 Μm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution. Agilent Bio SEC-5 HPLC columns are available in 100Å, 150Å, 300Å, 500Å, 1000Å and 2000Å. 
 
Column Phase Size Exclusion 
 
Packing Spherical, high purity, porous silica with a hydrophilic polymeric coating 
 
Pore Structure 100Å, 150Å, 300Å, 500Å, 1000Å, 2000Å  
 
Column Exclusion Limits (in Daltons)  
100Å MW range: 100 - 100,000 
150Å MW range: 500 - 150,000 
300Å MW range: 5,000 - 1,250,000 
500Å MW range: 15,000 - 5,000,000 
1000Å MW range: 50,000 - 7,500,000 
2000Å MW range: >10,000,000  
 
Operating Temperature Limit Recommended range: 10-30°C, maximum: 80°C  
 
Operating Pressure Limit Recommended operating pressure: 137 bar (2,000 psi) 
Maximum pressure: 240 bar (3,500 psi)  
 
Mobile Phase Compatability Recommended: 150 mM phosphate buffer, pH 7.0; other aqueous buffers with high and low salt can be used  
 
Working Flow Rate  
0.1 - 1.25 mL/min for 7.8 mm ID columns 
0.1 - 0.4 mL/min for 4.6 mm ID columns  
Bio MAb HPLC Columns
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pH Range 2 to 12
Additional Specifications The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies. Bio MAb columns are available in 1.7 Μm, 3 Μm, 5Μm, and 10 Μm sizes, providing higher resolution with the smaller particles. The Bio MAb column features a highly uniform, densely packed, weak cation exchange layer. This layer of the Bio MAb column is chemically bonded to the hydrophilic, polymeric coating of the Bio MAb column. 
 
Column Phase Weak Cation Exchange (carboxylate)  
 
Packing Non-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating and bonded with a uniform, weak cation exchange layer  
 
Particle Size 1.7, 3, 5, and 10 Μm  
 
Pore Structure Non-porous   
 
Operating Temperature Limit 80°C  
 
Column Hardware Operating Pressure Limit  
600 bar (8,700 psi) for stainless steel column hardware 
400 bar (5,800 psi) for PEEK column hardware 
 
Particle operating pressure limit 275 bar (4,000 psi) for 10 Μm particles 
413 bar (6,000 psi) for 5 Μm particles 
551 bar (8,000 psi) for 3 Μm particles 
689 bar (10,000 psi) for 1.7 Μm particles 
 
Mobile Phase Compatability Compatible with aqueous solution buffers, acetonitrile/acetone/methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate  
 
Working Flow Rate Typical 0.1-1.0 mL/min for a 4.6 mm ID column  
Bio Ion Exchange HPLC Columns
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pH Range 2 to 12
Additional Specifications Agilent Bio Ion Exchange HPLC columns are packed with polymeric, nonporous, ion exchange particles. Bio Ion Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins. The Bio IEX family offers strong cation exchange (SCX), weak cation exchange (WCX), strong anion exchange (SAX) and weak anion exchange (WAX) phases. All Bio Ion Exchange phases are available in 1.7, 3, 5 and 10 Μm non-porous particles sizes. 
 
Column Phase  
SCX (Strong cation exchange) – SO3H 
WCX (Weak cation exchange) – COOH 
SAX (Strong anion exchange) – N(CH3)3 
WAX (Weak anion exchange) – N(C2H5)2 
 
PackingNon-porous, poly(styrene divinylbenzene) (PS/DVB), grafted hydrophilic coating and bonded with a uniform, ion exchange layer 
 
Particle Size 1.7, 3, 5, and 10 Μm 
 
Pore Structure Non-porous 
 
Dynamic Binding Capacity  
SCX NP3: 53 mg/mL, SCX NP5: 38 mg/mL, SCX NP10: 20 mg/mL 
WCX NP3: 19 mg/mL, WCX NP5: 15 mg/mL, WCX NP10: 10 mg/mL 
SAX NP3: 35 mg/mL, SAX NP5: 28 mg/mL, SAX NP5: 17 mg/mL 
WAX NP3: 26 mg/mL, WAX NP5: 18 mg/mL, WAX NP3: 12 mg/mL 

Operating Temperature Limit 80°C 
 
Column Hardware Operating Pressure Limit  
600 bar (8,700 psi) for stainless steel column hardware 
400 bar (5,800 psi) for PEEK column hardware 
 
Particle Operating Pressure Limit  
275 bar (4,000 psi) for 10 Μm particles 
413 bar (6,000 psi) for 5 Μm particles 
551 bar (8,000 psi) for 3 Μm particles 
689 bar (10,000 psi) for 1.7 Μm particles 
 
Mobile Phase Compatability  
Compatible with aqueous solution buffers, acetonitrile/acetone/methanol and water mixtures. Commonly used buffers: phosphate, tris, MES and acetate. 
 
Working Flow Rate 
Typical 0.1-1.0 mL/min for a 4.6 mm ID column 

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Agilent BioHPLC Columns

Agilent BioHPLC Columns

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