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Thermo Scientific Accela

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Accela

Thermo Scientific Accela High Speed LC provides fast, efficient chromatographic separations over a range of flowrates and pressures for HPLC and UHPLC in a single system using the industry's only quaternary high pressure pump.  
  
Thermo Scientific chromatography instruments allow users to achieve shorter run times with excellent peak symmetry and superior data quality  
  
Accela is designed to optimize performance of sub-two micron particle columns, providing seamless operation from conventional LC pressures up to 1250 bar for U-HPLC separations of complex matrices.
Accela
Attributes
Optional Flow Rate 1 to 5000 µL/min
Detector UV/VIS Detector, RI Detector, PDA Detector,
Maximum Pressure 18130 psi
Standard Flow Rate 1 to 2000 µL/min
Additional Specifications Accela 600 Pump  
  
Minimum Flow Rate: 1.0µL/min (isocratic)  
Maximum Flow Rate: 5000µL/min  
Flow Rate Resolution: 0.1µL/min  
Flow Rate Accuracy: ±0.5% at set point or ±1µL/min whichever is greater  
Pressure Range: 0 to 600 bar (0 to 8,700psi)  
Pressure Resolution: 0.01 bar (0.15psi)  
Solvent Capacity: Quaternary valve system  
Delay Volume: 90µL  
Compositional Accuracy: ±0.50% at all operating flow rates and pressures  
Compositional Precision: <0.2% RSD  
Primary Piston Volume: 46µL  
Degasser Type: Integral, self-adjusting, 4 channel vacuum membrane (PTFE AF)  
Degasser Hold Volume: <500µL per channel  
Residual Pulsation: <0.5 bar amp. (typical)  
Pulse Dampener: No pulse dampener  
Dimensions: 18.5D x 14.17W x 7.08 in. H (47 x 36 x 18cm)  
Weight: 26.4 lb. (12kg)  
  
Accela 1250 Pump  
  
Minimum Flow Rate: 1.0µL/min (isocratic)  
Maximum Flow Rate: 2000µL/min  
Flow Rate Resolution: 0.1µL/min  
Flow Rate Accuracy: ±0.5% at set point or +1µL/min whichever is greater  
Pressure Range: 0 to 1250 bar (0 to 18,130psi)  
Pressure Resolution: 0.01 bar (0.15psi)  
Solvent Capacity: Quaternary valve system  
Delay Volume: 70µL  
Compositional Accuracy: ±0.50%  
Compositional Precision: <0.2% RSD  
Primary Piston Volume: 23µL  
Degasser Type: Integral, self-adjusting, 4 channel vacuum membrane (PTFE AF)  
Degasser Hold Volume: <500µL per channel  
Residual Pulsation: <0.5 bar amp. (typical)  
Pulse Dampener: No pulse dampener  
Dimensions: 18.5D x 14.17W x 7.08 in. H (47 x 36 x 18cm)  
Weight: 26.4 lb. (12kg)  
  
Accela Autosampler  
  
Vial Capacity: 200 vials with tray temperature control or three 96- or 384-well plates  
Variable Volume: Injection Precision: 1.0% RSD at 5µL and higher  
Minimum Sample Volume: 1µL can be injected from a 5µL sample  
Injection Volume: 25µL loop standard, up to 1000µL injections with larger loops and syringe  
Sample Carryover: <0.005% with UV detection (254nm) and a 1mL 100% MeOH needle wash  
Needle Height: Programmable in 0.1mm increments. Active vial bottom search selectable on/off  
Column Oven: 5 to 95°C ± 0.5°C selectable in 1°C increments  
Tray Temperature Control: 0 to 60°C ± 0.5°C selectable in 1°C increments  
Communications:   
Remote Inputs: Pump Ready and Inject Hold  
Remote Outputs: Pump Stop, Autosampler  
Ready, Inject, and Gradient Start  
4 Timed Events  
Sample Preparation: Capacity for four 15mL reagent vials for flushing, washing, sample dilution, and/or reagents for sample derivatization  
Ambient Environment: 10 to 40°C, 5 to 95% relative humidity, non condensing  
Dimensions: 19.68D x 14.17W x 14.57 in. H (50 x 36 x 37cm)  
Weight: 66.1 lb. (30kg)  
  
Accela Open Autosampler  
  
Vial Capacity:  
324 vials (2.0mL)  
Twelve 96- or 384-well plates  
Six 96- or 384-deep well plates  
Reproducibility: <1% RSD (partial loop)  
Sample Carryover: <0.003% chlorhexidine (with two wash solutions) and UV detection (257nm)  
Typical Clean Cycle Time: Less than 1 minute for Fast Cycle  
Tray Temperature Control: 4 to 40°C  
Communications: Remote Inputs, Pump Ready and Inject Hold  
Remote Outputs: Pump Stop, Autosampler Ready, Inject, and Gradient Start  
Syringe Size: 100µL, Optional syringe sizes available (10 to 5000µL)  
Maximum Operating Pressure: 18,000psi  
Ambient Environment: 4 to 40°C, 5 to 80% relative humidity, (non condensing)  
Dimensions: 19.68D x 14.17W x 14.57 in. H (50 x 36 x 37cm)  
Weight: 22 lb. (10kg)  
  
Accela PDA Detector  
  
Wavelength Range: 190 to 800nm at 1nm increment  
Wavelength Accuracy: ±1nm at 254nm and 640nm  
Wavelength Calibration: Using Holmium oxide solution  
Wavelength Resolution: 1.2nm (512 pixel array)  
Absorbance Non-Linearity: <5% at 2.0 AU at 257nm  
Absorbance Range: -2.0 to +4.0 AU, 20-bit resolution  
Drift: <1 mAU/hr after warm-up at 254nm at a stable temperature ±1°C  
Light Source: Pre-aligned Deuterium and Tungsten lamps  
Rise Time: User selectable: 0, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2, 5, or 10 sec  
Scan Rate: User selectable: 0.5, 1, 2, 4, 5, 10, 20, 40, or 80Hz  
Discrete Channels: Three wavelength selectable channels  
Short-Term Noise (50 mm flow cell):   
<6µAU/cm at 254nm at 5Hz (20Hz scan rate)  
<1.5µAU/cm at 254nm at 5Hz (40Hz scan rate); typical  
<0.3µAU/cm at 254nm at 5Hz (80Hz scan rate); typical  
Warm-up Time: 90 min. required to meet noise and drift specifications  
Cell Dimensions: 10mm, 2µL LightPipe flow cell; 50mm, 10µL LightPipe flow cell  
Cell Pressure Range: 0 to 1000psi  
Analog Outputs: 20-bit digital/analog conversion, unattenuated at 10mV/AU,100mV/AU, or 1.0V/AU  
Diodes: 512  
Diode Spacing: 1.2nm  
Dimensions: 18.5D x 14.17W x 7.08 in. H (47 x 36 x 18cm)  
Weight: 43 lb. (19.5kg)  
  
Accela UV/VIS Detector  
  
Wavelength Range: 190 to 800nm  
Deuterium Lamp: 190 to 365nm  
Tungsten Lamp: 366 to 800nm  
Wavelength Accuracy: ±1nm at 254nm and 656nm  
Wavelength Precision: <0.01nm  
Spectral Bandwidth: 6nm  
Absorbance Linearity: <5% up to 2.0 AU at 254nm  
Absorbance Range: 0.0005 AUFS to + 3.0 AUFS  
Drift: <1 mAU/hr at 254nm and 546nm using a 50mm LightPipe flow cell  
<0.1 mAU/hr at 254nm and 546nm using a 10mm standard analytical quartz flow cell  
Light Source: Pre-aligned Deuterium and Tungsten lamps  
Rise Time: User selectable: 0, 0.1, 0.2, 0.5, 1, 2, 3, 4, or 5 sec  
Scan Rate: User selectable: 4, 5, 6.67, 10 or 20Hz  
Discrete Channels: Two wavelength-selectable channels  
Warm-up Time: 90 min. required to meet noise and drift specifications  
Short-Term Noise:   
<6µAU/cm at 254nm, 2 sec rise time, single wavelength mode using a 50mm LightPipe flow cell  
<20µAU/cm at 254nm, 2 sec rise time, single wavelength mode using a 10mm standard analytical quartz flow cell  
Cell Dimensions: 10mm, 15 µL standard analytical; 50mm, 10 µL LightPipe; 10mm, 2 µL LightPipe  
Cell Pressure Range: 0 to 1000psi  
Dimensions: 18.5D x 14.17W x 7.08 in. H (47 ? 36 ? 18cm)  
Weight: 43 oz. (19.5kg)  
  
Accela RI Detector  
  
Recommended Flow Rate: 0.2 to 3.0mL/min  
Maximum Flow Rate: 10mL/min  
Linearity: <60 x 10-5 RIU  
Short-term Noise: 2.5 x 10-9 RIU  
Auto Zero Range: ±10 x 10-5 RIU  
Auto Zero Resolution: ±10 x 10-9 RIU  
Drift/Unit Time: 2 x 10-7 RIU/h  
Drift/Unit Temperature: 2 x 10-7 RIU/°C  
Integrator Output: 0 to 1V (512 x 10-5 RIU/FS)  
Recorder Output: 0 to 10mV  
Measuring Method: Deflection method  
Refractive Index Range: 1.00 to 1.75 RIU  
Response: FAST/STD/SLOW (3 steps)  
Flowcell Volume: 8µL  
Flowcell Pressure: 5kg/cm2 max  
Dimensions: 15.74D x 10.23W x 6.29 in. H (40 x 26 x 16cm)  
Weight: 39.6 lb. (18kg)

Features

      
  • Rapid gradient formation with extremely low delay volumes  
  • Maximum application flexibility with HPLC/U-HPLC in a single system  
  • Precise flow and gradients with proprietary Force Feedback Control (FFC) technology  
  • Streamlined integration across a full range of LC & MS detectors  
  
Accela 1250 Pump  
      
  • Accelerated method development time with quaternary solvent delivery  
  • HPLC & U-HPLC applications up to 1250 bar  
  • Extremely fast cycle with a 70µL delay volume  
  • FFC technology ensures consistent baseline reproducibility  
  
Accela 600 Pump  
      
  • Accelerated method development time with quaternary solvent delivery  
  • Greater application flexibility with flow rates up to 5mL/min at 600 bar  
  • Minimum gradient equilibration time with a 90µL delay volume  
  • FFC technology eliminates the need for pulse dampeners and reduces maintenance costs  
  
Accela Open Autosampler  
      
  • Maximum flexibility with unmatched reliability and robustness  
  • Customizable temperature controlled sample formats  
  • Integrated Dynamic Load and Wash capability resulting in extremely low carryover (0.003% chlorhexidine)  
  • Saves precious bench space with a customizable open frame architecture in a stackable design  
  
Accela Autosampler  
      
  • Optimized for high-throughput applications and sub two micron column particles  
  • Maximum reproducibility with isothermal injection and separation  
  • Greater application flexibility with multiple sample formats  
  • Superior robustness with a diamond coated high pressure valve Accela Photo Diode Array Detector  
  • Highest detection sensitivity with proprietary LightPipe technology  
  • Designed for U-HPLC separations with a 1cm path length, 2µL LightPipe flowcell  
  • Maximum peak resolution with fiberoptic beam shaper  
  • Easy maintenance with integrated wavelength validation  
  
Accela Photo Diode Array Detector  
      
  • Highest detection sensitivity with proprietary LightPipe technology  
  • Designed for U-HPLC separations with a 1cm path length, 2µL LightPipe flowcell  
  • Maximum peak resolution with fiberoptic beam shaper  
  • Easy maintenance with integrated wavelength validation  
  
Accela UV/Vis Detector  
      
  • Unlimited applications flexibility with externally mounted flow cells  
  • Simultaneous detection at two selectable wavelengths in the UV or the visible range of the spectrum  
  • Instrument versatility with two analog outputs  
  • Simple maintenance with pre-aligned deuterium and tungsten lamps  
  
Accela Refractive Index (RI) Detector  
      
  • High sensitivity HPLC detection  
  • Faster throughput with a low delay volume flowcell  
  • Automatic lamp brightness compensation for flowcell stains  
  • Optimum sensitivity and selectivity with Thermo Scientific columns  
  
  
Compatible with: Thermo Scientific Mass Spectrometers, Thermo Scientific Hypersil GOLD HPLC columns, ChromQuest CDS, Xcalibur MS, Atlas client/server CDS  
  
Recommended for: Pharmaceutical, Food Safety, Environmental, Forensics, Evidence Analysis, Drug Screening, Metabolite Analysis  
  
Compatible with: Thermo Scientific Atlas 8.2 client/server CDS; Thermo Scientific Hypersil GOLD HPLC columns; Thermo Scientific mass spectrometers  
  
Recommended for: Forensics, Evidence Analysis, Environmental, Biodiesel Analysis, Drug Screening, Metabolite Analysis

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Accela High Speed LC

Accela High Speed LC

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Q Exactive and Accela negative mode

I usually run lipids in the QExactive using Accela, but now I need to use it in negative mode. So, the solvents over the Accela all have FA, but the samples provided have no FA, just ACN and ISO (1:1). I calibrated the QExactive for negative...

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