Nikon A1R MP



Nikon’s A1R MP is a unique multiphoton imaging system featuring a high resolution galvanometer scanner and a high speed resonant scanner that is capable of frame rates from 30 fps at 512 x 512 pixels to as fast as 420 fps in band scan mode. New 4 channel non-descanned detectors with higher sensitivity, reduced dark current and broad spectral range allow for real time unmixing of closely spaced probes for accurate and high-contrast spectral imaging. This is especially important in multiphoton microscopy because of the overlap of emission spectra of probes and autofluorescence, which is often unavoidable when using a single laser line.
Microscope Type Confocal


Resonant Scanner Enables Imaging Up To 420fps  
Nikon's exclusive resonant scanner is capable of imaging a wide area at a much higher speed than a non-resonant scanner, making it possible for 420-fps imaging— the world's fastest using point scanning technology. The NDD*1 for multiphoton microscopy makes it possible to image fast and deep through the thickest specimens. Nikon's optical pixel clock system allows more stable and more evenly illuminated imaging— even at high speeds.  
Deep Imaging with High-sensitivity Non-Descanned Detector  
Nikon's newly developed 4-channel NDD for multiphoton microscopy allows imaging deep into a specimen. A detector* with very high sensitivity and a wider sensitive area than conventional models is placed close to the back aperture of the objective where image formation of the specimen takes place. This configuration improves the detection efficiency for scattered fluorescence, and enables the capture of signals from deep within a living organism more clearly and more reliably with higher S/N and less aberrations.  
Sharper, Brighter Imaging with New High NA Objectives  
The new water immersion objectives offer superior chromatic aberration correction throughout a wide wavelength range and high transmission covering from near-ultraviolet to near-infrared range, made possible by Nikon's proprietary Nano Crystal Coat*. CFI Plan Apo IR 60x WI with an NA of 1.27—the world's highest for a 60x water immersion objective—offers high-contrast imaging with excellent resolution and brightness.  
High-speed, High-precision Unmixing  
In addition to spectral detection and unmixing by the 32-channel detector, unmixing by the 4-channel NDD for multiphoton microscopy is also realized. Images captured with a resonant scanner can be unmixed, so clear and high-contrast images of areas deep within a thick specimen can be captured in ultrahigh speed.  
Multiphoton Laser Beam Alignment With a Single Click  
When the multiphoton laser wavelength or group velocity dispersion precompensation is changed, the multiphoton laser beam positional pointing may drift, resulting in uneven intensity of images, or a slight misalignment of fluorescence images that are produced by a visible light laser and a multiphoton laser.  
Since the multiphoton laser is invisible to the eye, the laser beam alignment has been difficult and potentially dangerous for users, especially at wavelengths longer than 800 nm. Nikon A1R MP's newly developed auto laser alignment function automatically corrects multiphoton laser misalignments with a single click.




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A1R MP alignment

Can anyone suggest a way to align the IR laser on the Nikon A1R MP system on a FN1 (upright) frame when you don't have the DS USB camera that the Nikon service techs use to do that alignment?  Is there a strategy to use the scanner itself...

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