Microplates for Cell-Based Assays
Cell-based assays have become the go-to technology for pharmaceutical, environmental, and toxicology labs. Cells are far less costly to maintain than test animals and don’t involve the ethical considerations. But the complexity of cell-based assays compared with chemical or biochemical assays presents distinct challenges to laboratory managers.
Balancing Assay Speed, Reading Sensitivity, and Culture Conditions
Interfering events wreak havoc with live assays. Cells’ natural fluorescence overlapping with detection signals is one example. Moreover, auto fluorescence varies unpredictably with cell health, which is a function of temperature, humidity, and gas composition.
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