How Does Temperature Affect a Compound’s Retention Time? | Labwrench eNews | LabWrench

February 16, 2016

Featured Video

Thermal Lab Beads replace ice in temperature controlled ice bucket

The Electric Ice Bucket, ICE-1200, is an accessory which can be attached to a temperature controlled base unit, the AHP-1200CPV Hot/Cold Plate. Peltier technology heats or cools the bath via a built in programmable PWM temperature controller. Watch the video here.

Recently Asked Questions

PerkinElmer/AAS 800 - Selenium woes
I am having trouble recovering Selenium spikes. Good spike and Second source recovery. Using Paladium and Magnesium nitrate modifier. Any suggestions? Advise this member.

Bio-Rad/MJ Mini Personal Thermal Cycler - Ready to update
I get this message on the screen and the machine is not responding. VERSION 1.2A READY TO UPDATE... 33789,253. Advise this member.

Waters/432 Conductivity Detector - Verifying detector
Following the instructions for this in the manual, I set the temp to 35deg, then allow to equilibrate for 3 hours, base range set at 200, filter time response to fast, and pump 1mM KCl at 1.2 mL/min, it says I should be getting an output reading of 147 +/- 5. I am getting a consistent reading of 121. Any advice or help with this matter is appreciated. Advise this member.

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Product of Interest

Arizona Instrument Jerome J505

The Jerome® J505 Mercury Vapor Analyzer is a portable fluorescence spectroscopy analyzer, which allows the detection cell to be simpler, smaller, lighter weight and more durable than competing spectroscopy instruments. The highly efficient optical cell requires less flow to purge the system, allowing the J505 to run at a lower flow rate minimizing sample dilution as found in competing spectroscopy instruments.

Read more about the Jerome J505 on the Arizona Instrument website.

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 PE Perkin Elmer HPLC 200 Series Chromatography Liquid Micro Pump

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Featured Blog

How Does Temperature Affect a Compound’s Retention Time?

Evaporation is the process in which a liquid slowly transfers from the condensed (liquid) phase to the gas phase—like the peaceful scene pictured above.

If temperature is increased, heat transfers more energy to the liquid—giving molecules the power to escape from the liquid’s surface. This rise in temperature therefore increases the transfer from liquid into the vapor phase.

Click here to read more from Phenomenex.

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Company News Hot Off The Press

BMG LABTECH introduces the PHERAstar® FSX - The new gold standard microplate reader for high throughput screening
The PHERAstar® FSX is based on an innovative optical design including three independent light sources including the next generation laser for TRF/TR-FRET capable of 60 flashes per second increasing throughput and precision of measurement in all plate formats. Read more.

Leica Biosystems and Advanced Cell Diagnostics Partner to Develop and Commercialize RNAscope-based Diagnostic and Companion Diagnostic Tests
Leica Biosystems, a global leader in anatomic pathology solutions and workflow automation, and Advanced Cell Diagnostics, Inc. (ACD), a world leader in RNA biomarker analysis for precision medicine, today announced a comprehensive partnership to develop and commercialize tissue-based diagnostic tests based on ACD's RNAscope in situ hybridization (ISH) assays on Leica Biosystems' BOND clinical advanced staining instruments. Read more.

Flexible Denaturing Gradient Gel Electrophoresis (DGGE) System
The next generation OmniPAGE VS20WAVE-DGGE from Cleaver Scientific is a flexible Denaturing Gradient Gel Electrophoresis (DGGE) system. Modular in design the system can be simply adapted to perform most single-base pair mutation detection techniques that are used in DNA mutation analysis. Read more.

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Thank you to ELGA for sponsoring our Water Purification category on LabWrench

ELGA is an integral part of Veolia Water Technologies, the world leader in water treatment.

ELGA focuses exclusively on water and its treatment. We continually contribute to the unique technical and scientific applications expertise developed during the last 75 years. ELGA is experienced in meeting the challenges that arise during the development, installation and servicing of single point-of-use purification systems as well as large projects involving consultation with architects, consultants and clients.

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Lab Equipment Questions for the Experts

Anton Paar/DMA Series - Error code
Can someone tell me what this code means and how I can fix it? Advise this member.

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Can you let me know what are the settings in keV and mA for the calibrations on the Bruker Tracer IV PDA & laptop. GQ15 GQ40 Thank-you, a tracer IV user (we have been using our own calibration with non-standard settings of keV and mA--and I want to use the Bruker built in calibrations) Advise this member.

Shimadzu/AA-6300 - Line search not found
In my AA6300 the line search is out of the wavelength recommended, and we can`t center it in the correct wavelength value. What can we do? Advise this member.

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