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Free Varian Atomic Absorption Spectrometer, SpectraAA400
The Bren School at University of California, Santa Barbara, has a Varian Atomic Absorption Spectrometer, SpectraAA400 that we would like to give away. The equipment functioned properly when last used. The unit includes a PSC-56, Programmable Sample Changer, and the PC to run the unit. You must pay for shipping costs. Respond here.
GC Derivatization Reagents with Hewlett Packard 5890 Series II
There are a few reagents used to analyze samples for amphetamine metabolites by GC-MS. These and other drug metabolites are often found by similar methods. Prior to analysis of these compounds found in urine, silyl derivatization is being performed in our lab to improve analyte volatility prior to analysis by GC-MS. We are using BSTFA (more info) and MSTFA (more info). These derivatization reactions are being conducted with and without TMCS (Trimethylchlorosilane). I am looking for a proven method for using these derivatization reagents (MSTFA, BSTFA, and TMCS) with isotope labels. Specifically, I am trying to compile literature on deuterium labelled varieties of these compounds. If you have experience with quantification of analytes with these derivatization reagents, and you know of good references, it would be much appreciated. Help this member.
Question regarding Applied Biosystems StepOne Real-Time PCR System curves
We want to do an absolute quantification of a certain gene, we already do the standard curve for these target and we want to know if there is a way to use these curve for quantify an unknown sample in a different run? or we have to run the curve every single time? Share your knowledge.
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