- Enables enrichment from both low and high amounts of input chromatin
- Delivers low backgrounds and high signal-to-noise ratios for ultra-sensitive detection
- Suitable for a wide range of input amounts - from 10,000 to 1,000,000 cells
EMD Millipore, the life science division of Merck KGaA of Darmstadt, Germany, today introduced Magna ChIP® HiSens chromatin immunoprecipitation kits for superior enrichment and detection of specific regions of chromatin-associated DNA. These new kits enable target chromatin enrichment from both low and high amounts of input chromatin while delivering low backgrounds, high signal-to-noise ratios, and ultra-sensitive detection that is compatible with all commonly used downstream analysis applications including qPCR, next generation sequencing, and microarray.
Two versions of this new chromatin immunoprecipitation kit are available. The Magna ChIP® HiSens chromatin immunoprecipitation kit includes reagents, buffers, and beads for ChIP as well as materials for chromatin preparation and isolation from either cultured cells or tissues. The EZ-Magna ChIP™ HiSens chromatin immunoprecipitation kit includes positive and negative control antibodies and a validated qPCR primer set, in addition to the standard kit's components.
Unique to these kits is a single buffer system used for all sonication, ChiP, and wash steps. This streamlined approach, plus their specialized blend of protein A/G magnetic beads, not only allows ChIP from a wider range of antibody isotypes than kits with protein A or G beads alone, it also delivers lower backgrounds and higher fold enrichment. Unlike other approaches the Magna ChIP® HiSens kits are designed to allow ChIP analysis by qPCR without additional clean-up, reducing the time required to perform the protocol along with eliminating the potential for sample loss during clean-up.
Using these kits, researchers can ChIP from a wide range of input amounts (10,000 to 1,000,000 cells or cell equivalents) and get excellent performance starting from either cells or tissues. Performance of the Magna ChIP® HiSens kit was benchmarked against that of other low input ChIP kits. After strictly following the protocols for all kits evaluated, the Magna ChIP® HiSens kit showed significantly higher performance as measured by fold enrichment and background levels than competitor kits.