With the TriStar² S Berthold Technologies introduces monochromator technology in its basic multi-label microplate reader. The TriStar² S is capable of these reading technologies
• UV/VIS absorbance
• Time-resolved fluorescence
• BRET and BRET²
TriStar2 S with multiple measurement technologies employs the new patent pending optical concept ALL-4-ONE, for the first time enabling luminescence, fluorescence and absorbance measurements with highest respective sensitivity as known from dedicated instruments:
• Less than 6 amol ATP per well
• Less than 0.3 fmol Fluorescein per well
Besides filters the instrument is equipped with a unique 3-D double monochromator with high blocking efficiency and high f-number (increased transmission) for filterless absorbance measurements and fluorescence excitation. The instrument is characterized by a new dual mode PMT detector with extremely low noise for both, fluorescence and luminescence measurements. While unsurpassed digital single photon counting technology is applied for luminescence, in fluorescence measurements a new triggered analogue operation mode with virtually no electronic noise has been implemented. Furthermore optical filters can be used for luminescence measurements enabling BRET (e.g. functional assays for GPCR research) and multi-coloured luciferase reporter gene applications.
The TriStar2 S can be fitted with up to 3 reagent injectors – based on the proven and most accurate JET technology – and temperature control for the microplate compartment. Two of the injectors have their tips in reading position enabling the measurement of extremely fast reactions. The reagent compartment at the front is easily accessible and may keep reagents cooled by the addition of crushed ice. Special reagent mounts provide a safe fixation of small reagent vials und ensure complete consumption of the reagents through inclination of the vial.
Thus, the TriStar² S microplate reader offers a multitude of possibilities and a wide range of applications. Measurements of enzyme activities, phagocytosis, Calcium flux, cell viability, apoptosis, immunoassays, protein and DNA concentrations, protein-protein interactions as well as spectral analysis are only a few of the many uses of the TriStar² S.
The unit is operated via the proven and intuitive ICE software. Single and multiple endpoint readings are possible as well as kinetic and scanning measurements. The data are displayed in numerical and graphical formats and may be exported into EXCEL or printed.
For increased sample throughput the TriStar² can be integrated into lab automation systems.