Cancer researchers now have a set of ThermoFisher Scientific TaqMan gene expression assays designed to detect fusion transcripts using real-time PCR. Certain fusion transcripts are considered
rare and are not well characterized; therefore, detecting these rare events using a single
technology can deliver ambiguous results. As such, researchers often employ an
orthogonal method of validation to confirm results. As a complementary solution to next-
generation sequencing (NGS) panels, including the Oncomine Focus Assay, the TaqMan
gene expression assays are designed to serve as an orthogonal validation method for
confirming NGS results.
Cancer researchers performing fusion transcript detection using an orthogonal method of
validation to confirm NGS results.
• Built from proven TaqMan Assays chemistry
• Performance guaranteed for all pre-designed assays.
• Consists of a single tube, which contains a probe, forward primer, and reverse
primer for each target for use in the simple, fast PCR workflow
• Use universal cycling conditions – can run any combination of assays in
parallel on a single real-time PCR instrument