Viobility Fixable Dyes from Miltenyi Biotec

The new ViobilityTM Fixable Dyes from Miltenyi Biotec allow the swift discrimination between live and apoptotic or dead cells by flow cytometry. The three dyes react with the primary amino groups of proteins. Optimized for various fluorescence channels, they are available from now on.

Miltenyi Biotec has developed the ViobilityTM Dyes for the verification of and differentiation between living and dead cells. The dyes react with the primary amino groups of proteins and can permeate the weakened cell membranes of apoptotic and dead cells. The fluorescent staining of dead cells is therefore up to 50-fold brighter than on living cells. This makes it possible to measure the viability of a cell by using a flow cytometer, regardless of whether fixed or unfixed cells are analyzed.

The three new fluorophores, which are derived from the Vio® Dye Family, are optimized for various fluorescence channels:

  • ViobilityTM 405/452 Fixable Dye (Ex.: 405 nm, Em.: 452 nm)
  • ViobilityTM 405/520 Fixable Dye (Ex.: 405 nm, Em.: 520 nm)
  • ViobilityTM 488/520 Fixable Dye (Ex.: 488 nm, Em.: 520 nm)

It is particularly in comparison with established dyes such as propidium iodide (PI) that the ViobilityTM Dyes can display their advantages. Firstly, they don’t have any toxic effects on living cells. Secondly, they show less spillover into other fluorescence channels. In addition, ViobilityTM Dyes facilitate higher flexibility in the design of antibody panels because they are available for various excitation and

All three dyes are available from Miltenyi Biotec in quantities sufficient for 100 or 500 tests.