RNA nanotechnology is gaining in popularity due to its simplistic and rational design for creating nanostructures for applications in medicine and therapeutics. RNA nanosquares were previously designed and constructed demonstrating the tunable size and stability of RNA as a nanoparticle delivery system. Dynamic light scattering (DLS) and atomic force microscopy (AFM) were utilized to characterize the size and shape of the squares verifying the molecular model and native PAGE assembly data. Here, we display the use of analytical ultracentrifugation (AUC) to further validate the size of the nanostructures, promoting the use of the instrument as an orthogonal tool for nanoparticle characterization and provide insights into applications where AUC provides distinct advantages for nanoparticle characterization over other sizing methods.
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