Caliber I.D. debuts the RS-G4, a new class of confocal microscope fusing extraordinary speed with multiple wavelengths for very large area imaging

Caliber I.D. launches the RS-G4, a new class of modular confocal microscope that delivers confocal’s expected high resolution and clean contrast while overcoming its limited scan areas.  Scanning large areas at lightning fast speeds, RS-G4 dramatically cuts the time required for efficient study of next-generation large format samples including brain slices, plant cross-sections, or large fields of cells or tissue critical for high-throughput screening.

Consider the attached image of a whole mouse brain (Figure 1).  The RS-G4 scanned and stitched 1050 fields to image this 12mm x 8mm area (approximately 0.5” x 0.25”) in just 270 seconds (4.5 minutes), approximately one-tenth the time required by other currently-available systems.  Yet, as demonstrated by the inset, the image retains all the confocal detail and resolution offered by the 40x/1.30NA oil immersion objective.

Figure 1: Whole mouse brain

The cornerstone of RS-G4’s innovative performance is its high-speed strip mosaic imaging. On the hardware side, continual stage movement is coordinated with the resonant scanning of the confocal microscope.  Simultaneously, a proprietary stitching algorithm assembles and aligns mosaic strips with pixel level resolution. Hardware and software control are integrated under an easy-to-use, intuitive GUI.  Adding a new laser launch opens opportunities for fluorescence excitation at 405nm, 488nm, 561nm, 640nm and, critical for deep tissue neuroscience, 785 nm.  In addition, the RS-G4 can collect large area 3D image stacks that can be readily imported by any conventional processing package such as Image J or Imaris.  Surprisingly, all of this capability comes in a small 18” x 15” footprint.      

“We at Caliber are thrilled to be launching this revolutionary product (the RS-G4) into the research market,” said Robert Kelley, Caliber I.D.’s Vice President of Sales.  “For researchers, this increased scan speed means greater productivity and shorter time to publication.”

Caliber I.D.’s RS-G4 with the new multi-laser launch will be running samples in BOOTH# 130 at the annual meeting of the Society for Neuroscience, November 12-16, in San Diego, CA.