Adeno-Associated Virus titer and aggregation characterization

In this application note, we describe a novel method to rapidly measure the size and concentration of Adeno-Associated Virus (AAV), utilizing the Nanoparticle Tracking Analysis (NTA) technique and a gold labelling method. By labelling AAV capsids with small gold nanoparticles, we could increase the amount of light scattered by each capsid, enabling rapid NTA characterization to determine the virus concentration and aggregation state.  NTA characterization of AAV was previously impossible without gold labelling.

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