A new imaging mode for the ZEISS LSM 8 family with Airyscan has been introduced. Their unique 32-channel GaAsP array detector captures more spatial information than traditional confocal microscopes. The new 2D Superresolution mode now uses this additional information to create an optical section of 0.2 Airy units (AU) and resolves structures down to 120 nanometer laterally in a single image.
The benefits for scientists
In the past, researchers had to acquire a stack of z-slices and subsequently deconvolve to get
optical sections thinner than one AU and enhance lateral resolution. Temporal resolution was
thus limited, and a prolonged light exposure of the sample was inevitable. Scientists can now use
the new 2D Superresolution mode to overcome this problem and perform gentle live cell imaging
experiments. They profit from very low light exposure, highly resolved structural information and
excellent signal-to- noise ratio.
The principle behind
ZEISS Airyscan is an area detector. Unlike traditional confocal microscopes which reject photons
from outside of the focal plane at a pinhole, ZEISS Airyscan detects all precious fluorescence
emission photons of 1.25 AU. Their information is then used to deliver higher sensitivity,
superresolution, and high acquisition speeds. The new 2D Superresolution mode takes
advantage of the fact that ZEISS Airyscan captures x, y and z information of the confocal point
spread function. A new exclusive processing algorithm uses this inherent spatial information
captured in a single image. It specifically distinguishes between photons originating from the
focal plane of 0.2 AU and photons from outside of this focal plane. In a traditional confocal
microscope, a researcher could only close the pinhole to 0.2 AU to attempt to achieve the same
optical sectioning. This would mean sacrificing many photons, even from the focal plane, thus
reducing signal-to- noise drastically.
Researchers can process both existing and new ZEISS Airyscan data with the new 2D