Liposomes have been used in drug discovery and drug delivery for some time, and the biophysical characterization of these systems and their payloads is critical to understanding and optimizing their fabrication and function. This study looks at optimal conditions for extruding liposomes as well as their stability under different conditions.
Our aim is to further educate the public about the intricacies of liposome formation and characterization as measured by Nanoparticle Tracking Analysis (NTA) from the NanoSight product range, Dynamic and Electrophoretic Light Scattering (DLS/ELS) from the Zetasizer product range, and Small-angle and Wide-angle X-ray scattering (SAXS/WAXS) from the X-ray analytical product range within Malvern Panalytical.
A broad range of characterization information and combination of both NanoSight and Zetasizer systems helped further optimize fabrication and understand the function of liposomes as well as labeling efficiency of fluorescent lipids. NTA through NanoSight provided number-based high resolution sizing, accurate distribution profiles, concentration (particles/mL), and fluorescence measurements. DLS provided excellent reproducibility, mean size and PDI measurements over a broad range and non-invasive trend analysis. ELS provided zeta potential as a functionality and stability metric of particles. SAXS allowed to investigate the bilayer stacking in MLVs and from WAXS data the order of the alkyl chains (gel phase vs. liquid phase) could be studied as a function of sample temperature.
January 23 2018 - January 23 2018
10:30 - 11:30
(GMT-05:00) Eastern [US & Canada]
Webinar - Live
Nanoparticle Tracking Analysis
Ragy Ragheb is a Technical Specialist at Malvern Panalytical specializing in the NanoSight and Zetasizer product ranges. Ragy received his Ph.D. in Macromolecular Science and Engineering from Virginia Polytechnic Institute and State University and completed his post-doctoral work in Biomedical Engineering at Yale University. His research focused on the fabrication and characterization of several synthetic and metal-based nanoparticle systems for theranostic and drug delivery applications.
Joerg Bolze holds a degree in chemistry and a doctorate in colloid science, both obtained from Karlsruhe University in Germany. In 2006 he joined PANalytical in the Netherlands, a leading supplier of instrumentation for X-ray diffraction and X-ray fluorescence analysis. As an applications specialist for small-angle X-ray scattering he was involved in the development of this technique on a multi-purpose X-ray diffractometer platform. He is now a product manager for nanomaterials at PANalytical. During 1997-2001 he worked as a Postdoctor at leading research institutes in Japan and Korea, where he applied various X-ray analytical techniques at synchrotron radiation facilities. Later he joined INTERPOLYMER Sàrl in France where he worked as an applications chemist on emulsion polymers as binders in various types of specialty paint and lacquer formulations.
Who should attend?
Anyone interested in characterizing both biological and synthetic nanoparticles.
What will you learn?
The benefits and complementary nature of both NanoSight (Nanoparticle Tracking Analysis) and Zetasizer (Dynamic and Electrophoretic Light Scattering) in characterizing liposomes.