Nanosized plasma membrane vesicles secreted by cells into bodily fluids are known to have an important role in intracellular communication. These little messengers, called exosomes or extracellular vesicles (EVs), are undergoing intensive research to prove their potential uses within diagnostics and as therapeutics. This research often involves characterization of a large number of exosome samples. Reliable physical characterization throughout the exosomes development workflow is crucial to fully understanding their formation, pathways, stability and responses to changing conditions.