Olympus - cell^TIRF

Manufactured by  Olympus
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The Olympus cell^TIRF illuminator offers four motorized channels for simultaneous image capture. Intuitive software...

The Olympus cell^TIRF illuminator offers four motorized channels for simultaneous image capture. Intuitive software control of TIRF parameters allows instant setting and confirmation of TIRF angle and seamless transition back and forth to widefield fluorescence. With the cell^TIRF illuminator, each laser wavelength is optimally focused and each angle is individually set, allowing different wavelengths to have the same penetration depth. Users can preset calculated penetration depths for all lasers with a single mouse click; the system will individually adjust each laser’s angle to simultaneously capture TIRF information from all channels.
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Features of cell^TIRF
  • The only 4 line system with true simultaneous acquisition     
  • Motorized control of TIRF angles     
  • Precise adjustment of incident angle     
  • Software calculation of penetration depth     
  • Software based laser attenuation     
  • Integrated Point FRAP optics for 1st laser line     
  • Seamless widefield/TIRF switching     
  • Four dedicated TIRF lenses, worlds highest NA of 1.65 as well as the unmatched 150x objective     
  • Includes 450nm - 640nm laser systems     
  • Flexibility to select your optimal wavelength     
  • TIRF and widefield or 100% widefield with 340nm Transmission     
  • With 25mm filter access for each laser line     
  • Adjustable field stop for each laser line     
  • Rigid single billet Aluminum frame     
  • Single molecule and membrane research     
  • Fully adjustable field stop for each laser line
General Specifications
Microscope TypeFluorescence
Additional Specifications

TIRF illuminator      
Available laser Systems: 405nm, 445nm, 473nm, 488nm, 515nm, 532nm, 561nm, 594nm, 640nm     
Laser coupling: FC or FC8 connector     
Field Number: FN13     
Illuminator Body: Single Billet Aluminum     
Safety: Integrated Interlock     
     
Laser systems      
Wavelengths: 405nm, 445nm, 473nm, 488nm, 491nm, 515nm, 532nm, 561nm, 594nm, 640nm     
Power: Laser system available up to 100mW     
Attenuation: Software control of laser intensity     
Imaging Shutter: High speed 1msec TTL shutters     
Safety: Integrated interlock     
     
Epi-Fluorescence      
Light source: 100W Mercury, 75W Xenon, Metal halide.     
Selection: TIRF/Brightfield or Brightfield only with 340nm transmission     
Field number: FN22     
TIRF Objectives: 60X Objectives      
APON60XOTIRFM: APO 60X, NA 1.49, WD 0.1mm, Correction collar     
UAPON100XTIRF: UAPON 100X, NA 1.49 WD 0.09mm, Correction collar     
APO100XO-HR-SP: APO 100X, NA 1.65, WD 0.10mm     
UAPON150XOTIRF: UAPO 150X, NA 1.45,WD 0.07mm, Correction collar     
     
Immersion Oil      
Type-F: Low Auto Fluorescence Immersion oil, Low odor-MSDS available     
High Refractive Index: RI 1.78 Immersion oil for use with AP0100XO-HR-SP 1.65NA 100X objective, Low odor-MSDS available     
     
Coverslips      
High Refractive Index: RI 1.78 glass coverslips for use with AP0100XO-HR-SP 1.65NA 100X objective     
     
Compatible microscopes: IX71, IX81 and IX81-ZeroDrift 2

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