Varian - Cary 50
This award-winning instrument represents the budget end of the Cary line and takes spectroscopy into the next era of design.
The innovative design of the Cary 50, which incorporates a Xenon flash lamp, enables it to offer many advantages over traditional UV-Vis spectrophotometers.
The Cary 50 is controlled by the new Cary WinUV software. This Windows based software features a modular design which makes it easy to use. And of course, the Cary 50 can be fitted with the full range of accessories to provide extra capabilities.
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Features of Cary 50
- The maximum scan rate is 24 000 nm per minute. That means you can scan the whole wavelength range of 190-1100 nm in less than 3 seconds.
- With a data collection rate of an impressive 80 points per second you'll have all the information you need about your kinetics assay.
- The Cary 50 can measure samples up to 3 Abs so you won't have to dilute as often.
- The Xenon lamp flashes only when acquiring a data point, unlike a diode array which exposes the sample to the whole wavelength range with each reading, causing degradation of photosensitive samples.
- As the Xenon lamp is very intense, the Cary 50 can use a beam splitter without the loss in energy causing excessive photometric noise. The beam splitter allows simultaneous reference beam correction, so peaks will not shift as the scan speed changes.
- The Cary 50's super-concentrated beam makes it ideal for fiber optic work.
- The light beam is narrow and very intense, so even if you are using microcells you will still get excellent noise performance.
- The Cary 50 is unaffected by room light. You can operate with the sample compartment open or closed, you won't notice the difference.
General Specifications
| Bandwidth | Fixed at 1.5 nm |
| Optics | Single Beam |
| Photodetector | Photodiode |
| Photometric Range | ± 3.3 Abs |
| Sample Compartment | (WxDxH): 130 mm x 523 mm x 123 mm Note that sample compartment can be left open during measurement due to room light immunity of Cary 50. |
| Scanning Speed | Maximum 24000 nm/min |
| Stray Light | At 198 nm (12 g/L KCl, TGA & BP/EP method): </= 1%; At 220 nm (10 g/L NaI ASTM method): </= 0.05%; At 370 nm (50 mg/L NaNO2): </= 0.05% |
| Depth | 590 mm |
| Height | 205 mm |
| Width | 500 mm |
| Power Requirements | Instrument draws maximum of 26 W of power from the host PC power supply. The power rating is +5 V DC <1 A, +12 V DC <1.5 A, -12 V DC <0.25 A. The Cary 50 interface card fits into a standard ISA slot in the host PC and requires a standard PC internal hard disk power supply connector. Operation of motor driven accessories may increase the +12 V requirement by a further 2 A (24 W maximum). The host PC must be certified to standard IEC 60950 or equivalent. |
| Weight | 21 kg |
Additional Specifications
Temperature Range: Between 853 and 2133 m altitude: 10–25 °C, 8–80% relative humidity, non-condensing
Grating: Holographic, 27.5 x 35 mm, 1200 lines/mm, blaze angle 8.6° at 240 nm
Beam splitting system; Beam splitter
UV-Vis limiting resolution: = 1.5 nm
Wavelength accuracy: ± 0.5 nm at 541.94 nm
Wavelength reproducibility: ± 0.1 nm
Photometric accuracy (Abs):
Using NIST 930D filters at 1 Abs ± 0.005
At 0.2, 0.5 & 0.75 Abs (14.2% w/v KNO3, TGA method) ± 0.01
0.292 to 0.865 Abs (60.06 mg/L K2Cr2O7, BP method) ± 0.01
Photometric display: ± 9.9999 Abs, ± 200.00 %T
Photometric reproducibility (Abs):
Using NIST 930D filters, at 465 nm, 2 s SAT
Maximum deviation at 1 Abs < 0.004
Standard deviation for 10 measurements < 0.00050
Using NIST 930D filters, at 546.1 nm, 2 s SAT
Maximum deviation at 0.5 Abs < 0.003
Standard deviation for 10 measurements < 0.0030
Photometric stability (Abs/hour): After 30 minute warm up, 500 nm, 10 s SAT < 0.0004
Photometric noise (Abs, RMS):
500 nm, 1 s SAT At 0 Abs < 0.0001
At 1 Abs < 0.0005
At 2 Abs < 0.005
260 nm, 1 s SAT At 0 Abs < 0.00015
Baseline flatness (Abs): 200 to 850 nm, smooth 21 filter applied, baseline corrected ± 0.001
Access: Top and front
Signal averaging: 0.0125 to 999 seconds
Slew rate: 24000 nm/min
Data interval: 0.15–5.0 nm
Repetitive scanning: 4800, Maximum number of cycles: 999, Maximum cycle time (min): 9999
Data collection rate: (kinetic studies) points per min per cell
1 cell = 4800, 6 cell = 6, 12 cell = 3, 18 cell = 2
6 cells, 0.0375 SAT 0.38 s dwell time = 40 to 50
12 cells, 0.0375 SAT 0.38 s dwell time = 20 to 30
18 cells, 0.0375 SAT 0.38 s dwell time = 10 to 20
Temperature monitors: Temperature probe inside cuvette (using the Temperature Probe Accessory)
Minimum sample volume: Approximately 5.0 µL
Warranty: Twelve (12) months, though this may vary according to locations.