Esco Technologies - Airstream PCR Cabinets
Manufactured by Esco Technologies
The Proven Solution for Contaminant-Free PCR
Polymerase Chain Reaction* (PCR) is a process where millions of copies of DNA are amplified from a single copy, or low copy number template. This reaction is fundamental to almost all applications requiring a high copy number of starting material and is used in all laboratories working with DNA and RNA.
Because of the high copy number generated during PCR, it is essential to prevent possible contamination of the PCR reaction. Precautions must be taken during the sample and reagent preparation steps to minimize this risk. In addition to good laboratory practice, the ideal PCR laboratory should consist of three areas, each isolated from the other. Reagents should be prepared in the reagent preparation area and transferred to the sample preparation area, through a pass box, or inside closed containers. After preparation of the final reaction mix, the tubes should be transferred to the amplification area, again through a pass box or in a closed container. The PCR amplification and results analysis takes place in this area.
To guarantee contaminant-free samples, it is essential to work in an environment where the air quality is controlled. This should form part of the equipment in the sample preparation area.
Because of the high copy number generated during PCR, it is essential to prevent possible contamination of the PCR reaction. Precautions must be taken during the sample and reagent preparation steps to minimize this risk. In addition to good laboratory practice, the ideal PCR laboratory should consist of three areas, each isolated from the other. Reagents should be prepared in the reagent preparation area and transferred to the sample preparation area, through a pass box, or inside closed containers. After preparation of the final reaction mix, the tubes should be transferred to the amplification area, again through a pass box or in a closed container. The PCR amplification and results analysis takes place in this area.
To guarantee contaminant-free samples, it is essential to work in an environment where the air quality is controlled. This should form part of the equipment in the sample preparation area.
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Features of Airstream PCR Cabinets
- True vertical laminar airflow with a large filter covering the entire work zone within the cabinet for better protection against cross contamination, as compared to conventional dead-air boxes.
- High-quality polyester pre-filter and main HEPA filter with a typical efficiency of 99.9998% at 0.3 microns provide the best product protection in the world; typical ULPA filter life span is more than 3 years depending on operating conditions and total number of hours in usage.
- Esco Sentinel™ Microprocessor Control provides users flexibility and full configurability according to the requirements of the user.
- Admin PIN can be set on the control to restrict unauthorized access to menu parameters.
- UV decontamination cycle is fully programmable using the Microprocessor keypad; a timer will turn off the UV lamp after the set period of time.
- Unique pre-filter change alarm and UV lamp replacement alarm make the cabinet the safest and the most user-friendly PCR cabinet on the market.
Airflow
- During operation, room air is drawn through the top of the cabinet via a pre-filter, with 85% arrestance, trapping larger particles and increasing the life of the main filter.
- The air is then forced evenly through the main HEPA filter resulting in a unidirectional stream of clean air that is projected vertically over the internal work zone. All airborne contaminants are flushed and diluted, resulting in a particulate-free work environment.
- The purified air then leaves the main work chamber across the open front of the cabinet.
- The average airflow velocity of 0.30 m/s (60 fpm) in PCR-3A and PCR-4A models ensures that cleanliness is maintained in the work zone with 35-38 air changes per minute.
General Specifications
There are no General Specifications available.
Additional Specifications
Standards Complied: IEST-RP-CC002.2, IEC61010-1, EN61010-1
Pre-Filter: Washable polyurethane fibers with 85% arrestance
Construction (Work Zone): 1.2mm (0.05
Series Modals
Esco Technologies - PCR-3A_
[+] View Model SpecificationsDepth | 40.7 in |
Height | 37.4 in |
Internal Depth | 36.8 in |
Internal Height | 21.7 in |
Width | 24.3 in |
Internal Width | 21.2 in |
Power Requirements | Three versions available |
Lighting | Fluorescent/UV |
Noise Level | 53 dB |
Construction | Electrogalvanised steel with white oven-baked epoxy powder-coated finish |
Face Velocity (FPM) | 60 |
Filter Type | HEPA |
Fume Hood Type | PCR Enclosure |
Additional Model Specifications | Laminar Flow Nominal size: 3' Standards Complied: IEST-RP-CC002.2, IEC61010-1, EN61010-1 Pre-Filter: Washable polyurethane fibers with 85% arrestance Fluorescent lamps intensity: >975 Lux (>91 foot candles) UV lamp: 253.7 nanometer 15-watt UV lamp Construction: Main body: Electrogalvanised steel with white oven-baked epoxy powder-coated finish. Coated with Esco Isocide antimicrobial coating. Work Zone: 1.2mm (0.05") 18 gauge stainless steel grade 304 Shipping Dimensions, Maximum*: 44.5"D x 28.9"W x 45.3"D Shipping Volume, Maximum*: 0.95 m3 (34 ft3) Shipping Weight, Maximum*: 123 kg (271 Lbs) Power supply**: PCR-3A1: 220-240 V, AC, 50 Hz PCR-3A2: 110-130 V, AC, 60 Hz PCR-3A3: 220-240 V, AC, 60 Hz * Cabinet only; excludes optional stand. ** Additional voltages may be available; contact Esco for ordering information. |
Esco Technologies - PCR-4A_
[+] View Model SpecificationsDepth | 52.8 in |
Height | 37.4 in |
Internal Depth | 48.8 in |
Internal Height | 21.7 in |
Width | 24.3 in |
Internal Width | 21.2 in |
Power Requirements | Three versions available |
Lighting | Fluorescent/UV |
Noise Level | 62 dB |
Construction | Electrogalvanised steel with white oven-baked epoxy powder-coated finish |
Face Velocity (FPM) | 60 |
Filter Type | HEPA |
Fume Hood Type | PCR Enclosure |
Additional Model Specifications | Laminar Flow Nominal size: 4' Standards Complied: IEST-RP-CC002.2, IEC61010-1, EN61010-1 Pre-Filter: Washable polyurethane fibers with 85% arrestance Fluorescent lamps intensity: >1230 Lux (>114 foot candles) UV lamp: 253.7 nanometer 30-watt UV lamp Construction: Main body: Electrogalvanised steel with white oven-baked epoxy powder-coated finish. Coated with Esco Isocide antimicrobial coating. Work Zone: 1.2mm (0.05") 18 gauge stainless steel grade 304 Shipping Dimensions, Maximum*: 55.9"D x 28.7"W x 45.3"D Shipping Volume, Maximum*: 1.18 m3 (42 ft3) Shipping Weight, Maximum*: 140 kg (309 Lbs) Power supply**: PCR-4A1: 220-240 V, AC, 50 Hz PCR-4A2: 110-130 V, AC, 60 Hz PCR-4A3: 220-240 V, AC, 60 Hz * Cabinet only; excludes optional stand. ** Additional voltages may be available; contact Esco for ordering information. |