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  5. Gilson - 350
This product is Discontinued

Gilson - 350

Manufactured by  Gilson
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Gilson’s 350 Micro Pump provides precise solvent delivery for nano to micro LC that delivers accurate and reproducible gradients from 300 nL/min.

Gilson’s 350 Micro Pump provides precise solvent delivery for nano to micro LC that delivers accurate and reproducible gradients from 300 nL/min. to 50 µL/min. The 350 is capable of delivering true micro flow rates without requiring a flow splitter.
  • Modular, binary pumping system with independent micro mixer ptimizes separation with nano, capillary, and micro columns for 1-D, 2-D, and LC/MS/MS applications
  • Splitless flow delivery eliminates the inaccuracy of flow splitters, while dramatically reducing solvent consumption
  • Dual-piston pump with patented magnetic coupling ensures precise, accurate flow rates from 300 nL/min. to 50 µL/min.
  • Quick pressurization protocol allows for fast system startup and column stabilization
  • Pump head seal wash permits the use of buffered solvents with increased confidence and decreased maintenance
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    Features of 350
    • Modular, binary pumping system with independent micro mixer optimizes separation with nano, capillary and micro columns for 1-D, 2-D and LC/MS/MS applications    • Splitless flow delivery eliminates the inaccuracy of flow splitters, while dramatically reducing solvent consumption    • Dual-piston pump with patented magnetic coupling ensures precise, accurate flow rates from 300 nL/min to 50 µL/min    • 60-nL mixer for fast gradient response times    • Pump head seal wash permits the use of buffered solvents with increased confidence and decreased maintenance
    General Specifications
    Depth 13 in
    Height12 in
    Pump Max. Pressure4350 psi
    Width 10 in
    Power Requirements 100–120 or 220–240V; 50–60 Hz
    Flow Accuracy 1 % (5–50 µL/min)
    Standard Flow Rate 0.3 to 50 µL/min
    Flow Delivery Double Piston
    Weight33 lbs
    Additional Specifications

    [u]Pump Options [/u]  
    Isocratic pump (350D): electrically-actuated valves, 300 nL/min to 50 µL/min, 30 MPa (300 bars, 4,350 psi), micro degasser  
    Gradient pumping system (350D + 350 + gradient pumping kit): electrically-actuated valves, 300 nL/min to 50 µL/min, 30 MPa (300 bars, 4,350 psi)  
    Flow Rate Gradient: 0.3–50 µL/min (4,350 psi)  
    Isocratic: 0.15–50 µL/min (4,350 psi)  
    Selectable units: nL/min or µL/min  
    Adjustment increment: 0.01 µL/min  
    Repeatability: <0.5% for 5–50 µL/min; <1% for 0.5–5 µL/min   
    Adjustment increment: 0.1% B for 1–50 µL/min; 1% B below 1 µL/min  
    Dwell volume/Effective mixing volume: 60 nL (up to 5 µL/min); 3 µL (above 5 µL/min)  
    Gradient linearity range: 2% at 1% B; 97% at 100% B

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